LRRN3 CRISPR/Cas9 KO Plasmid (m): sc-421473
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRRN3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LRRN3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRRN3 CRISPR/Cas9 KO Plasmid (m) | sc-421473 | 20 µg | $397.00 |
Overview
Lrrn3 encodes leucine-rich repeat neuronal protein 3 (LRRN3), a type I transmembrane protein enriched in neural tissues and implicated in cell–cell recognition, neurite extension, and synapse-associated signaling. Through its extracellular LRR domains, LRRN3 is thought to contribute to adhesion-like processes and coordination of neuronal differentiation programs, influencing network formation and plasticity. Expression patterns also link LRRN3 to immune cell states, where it is used as a marker of T cell maturation and functional exhaustion, suggesting roles in activation-associated transcriptional remodeling. Dysregulated Lrrn3 expression has been reported across neurodevelopmental and neurodegeneration-adjacent contexts and in immune-oncology datasets, supporting its use in mechanistic studies of neural and immune signaling.
LRRN3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lrrn3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lrrn3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lrrn3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LRRN3 protein expression.
This CRISPR knockout system enables efficient generation of Lrrn3-deficient cell models for investigation of LRRN3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lrrn3 exon(s) critical for LRRN3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lrrn3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LRRN3 CRISPR/Cas9 KO Plasmid (m) and LRRN3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lrrn3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LRRN3 HDR Plasmid (m) and LRRN3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lrrn3 homology arms to support homology-directed repair at defined Lrrn3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.