LRRN1 CRISPR/Cas9 KO Plasmid (m): sc-421471
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRRN1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LRRN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRRN1 CRISPR/Cas9 KO Plasmid (m) | sc-421471 | 20 µg | $397.00 |
Overview
Leucine rich repeat neuronal 1 (LRRN1; Lrrn1) encodes a single-pass membrane protein enriched in neural tissues and implicated in cell–cell interactions and neurite-associated processes. Through its extracellular leucine-rich repeat domains, LRRN1 is thought to influence adhesion-dependent signaling, neuronal differentiation, and tissue patterning during development, with links to pathways governing membrane organization and synaptic or axon guidance programs. Altered LRRN1 expression has been reported in multiple cancer-related transcriptomic datasets, supporting investigation of its contributions to proliferation, migration, and differentiation states without implying clinical utility. In mouse systems, Lrrn1 perturbation provides a tractable model to examine how LRRN1 shapes developmental phenotypes and signaling outputs in neurons and other LRRN1-expressing cell types.
LRRN1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lrrn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lrrn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lrrn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LRRN1 protein expression.
This CRISPR knockout system enables efficient generation of Lrrn1-deficient cell models for investigation of LRRN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lrrn1 exon(s) critical for LRRN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lrrn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LRRN1 CRISPR/Cas9 KO Plasmid (m) and LRRN1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lrrn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LRRN1 HDR Plasmid (m) and LRRN1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lrrn1 homology arms to support homology-directed repair at defined Lrrn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.