LRP16 CRISPR/Cas9 KO Plasmid (h): sc-405616
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRP16 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LRP16 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRP16 CRISPR/Cas9 KO Plasmid (h) | sc-405616 | 20 µg | $397.00 |
Overview
MACROD1 encodes LRP16, a nuclear macrodomain-containing protein that binds ADP-ribose and functions as an eraser of mono-ADP-ribosylation on target proteins. LRP16 participates in regulation of transcriptional programs and chromatin-associated processes, including crosstalk with nuclear hormone receptor signaling and PARP-dependent ADP-ribosylation pathways that influence DNA damage responses. Through these activities, MACROD1 can impact cell-cycle control, stress signaling, and maintenance of genomic stability. Altered MACROD1/LRP16 expression or function has been reported in multiple tumor contexts and is frequently investigated in relation to proliferation, survival signaling, and transcriptional rewiring.
LRP16 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MACROD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MACROD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MACROD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LRP16 protein expression.
This CRISPR knockout system enables efficient generation of MACROD1-deficient cell models for investigation of LRP16 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MACROD1 exon(s) critical for LRP16 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MACROD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LRP16 CRISPR/Cas9 KO Plasmid (h) and LRP16 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MACROD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LRP16 HDR Plasmid (h) and LRP16 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MACROD1 homology arms to support homology-directed repair at defined MACROD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.