Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LRF/Pokemon CRISPR/Cas9 KO Plasmid (m): sc-421462
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRF/Pokemon CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LRF/Pokemon genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRF/Pokemon Antibody (13E9): sc-33683
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRF/Pokemon CRISPR/Cas9 KO Plasmid (m) | sc-421462 | 20 µg | $397.00 |
Overview
Zbtb7a encodes LRF/Pokemon, a BTB/POZ zinc-finger transcription factor that regulates lineage commitment, proliferation, and survival through sequence-specific DNA binding and recruitment of corepressor complexes. In hematopoietic and immune contexts it helps coordinate B- and T-cell developmental programs, influences erythroid maturation, and modulates differentiation-associated transcriptional networks. LRF/Pokemon intersects with pathways controlling cell-cycle checkpoints and stress responses, including functional antagonism of ARF–p53 tumor suppressor signaling, and it can reshape chromatin states at target loci. Altered Zbtb7a activity has been implicated in oncogenic transcriptional programs and abnormal differentiation phenotypes in models of hematologic and solid malignancies, making it a relevant node for mechanistic studies of tumorigenesis and cell fate control.
LRF/Pokemon CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zbtb7a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zbtb7a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zbtb7a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LRF/Pokemon protein expression.
This CRISPR knockout system enables efficient generation of Zbtb7a-deficient cell models for investigation of LRF/Pokemon signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zbtb7a exon(s) critical for LRF/Pokemon function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zbtb7a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LRF/Pokemon CRISPR/Cas9 KO Plasmid (m) and LRF/Pokemon CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zbtb7a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LRF/Pokemon HDR Plasmid (m) and LRF/Pokemon HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zbtb7a homology arms to support homology-directed repair at defined Zbtb7a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.