LRF/Pokemon Antibody (13E9): sc-33683

LRF Antibody (13E9) is an Arm. hamster monoclonal IgG antibody that detects LRF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). LRF (13E9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LRF protein, also known as leukemia/lymphoma related factor, plays a crucial role in regulating gene expression and is involved in cell differentiation processes. LRF functions as a transcription factor containing a poxvirus zinc finger (POZ) domain, essential for protein-protein interactions, and Kruppel-type zinc fingers that facilitate DNA binding. This unique structure enables LRF to influence the transition from cellular proliferation to terminal differentiation, particularly during preadipocyte to adipocyte differentiation. LRF′s ability to associate with active chromatin and stimulate transcription of the HIV-1 long terminal repeat underscores its importance in both normal cellular functions and viral pathogenesis. Studies have shown that 3T3L1 cells stably overexpressing LRF exhibit reduced DNA synthesis and downregulation of key cell cycle regulators such as cyclin A, cyclin-dependent kinase 2, and p107, highlighting LRF′s significant role in modulating cell cycle progression and differentiation.

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LRF/Pokemon Antibody (13E9) References:

1. Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis.  |  Kukita, A., et al. 1999. Blood. 94: 1987-97. PMID: 10477728
2. FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles.  |  Pendergrast, PS., et al. 2002. Mol Biol Cell. 13: 915-29. PMID: 11907272
3. POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1.  |  Lee, DK., et al. 2002. J Biol Chem. 277: 26761-8. PMID: 12004059
4. Flexible DNA binding of the BTB/POZ-domain protein FBI-1.  |  Pessler, F. and Hernandez, N. 2003. J Biol Chem. 278: 29327-35. PMID: 12750370
5. Role of the POZ zinc finger transcription factor FBI-1 in human and murine adipogenesis.  |  Laudes, M., et al. 2004. J Biol Chem. 279: 11711-8. PMID: 14701838
6. Role of the proto-oncogene Pokemon in cellular transformation and ARF repression.  |  Maeda, T., et al. 2005. Nature. 433: 278-85. PMID: 15662416
7. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair.  |  Liu, XS., et al. 2015. Nat Commun. 6: 8325. PMID: 26446488
8. LRF acts as an activator and repressor of the human β-like globin gene transcription in a developmental stage dependent manner.  |  Kang, J., et al. 2019. Biochem Cell Biol. 97: 380-386. PMID: 30427207
9. The transcription factor LRF promotes integrin β7 expression by and gut homing of CD8αα+ intraepithelial lymphocyte precursors.  |  Nie, J., et al. 2022. Nat Immunol. 23: 594-604. PMID: 35354951
10. Leukemia/lymphoma-related factor (LRF) or osteoclast zinc finger protein (OCZF) overexpression promotes osteoclast survival by increasing Bcl-xl mRNA: A novel regulatory mechanism mediated by the RNA binding protein SAM68.  |  Xu, X., et al. 2022. Lab Invest. 102: 1000-1010. PMID: 35474350
11. LRF Promotes Indirectly Advantageous Chromatin Conformation via BGLT3-lncRNA Expression and Switch from Fetal to Adult Hemoglobin.  |  Chondrou, V., et al. 2022. Int J Mol Sci. 23: PMID: 35806029
12. Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene.  |  Davies, JM., et al. 1999. Oncogene. 18: 365-75. PMID: 9927193

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LRF/Pokemon Antibody (13E9) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-LRF/Pokemon Antibody (13E9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LRF/Pokemon Antibody (13E9) Specifications

Alternate Product Name: LRF

Product Clone Name: 13E9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids: 1-20

Epitope Target: LRF

Epitope Species: human

Product Reactivity/Detection: LRF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for LRF/Pokemon Antibody (13E9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin, X: TransCruz Reagent