LRF/Pokemon Antibody (H-6): sc-393012

LRF Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects LRF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LRF, also known as leukemia/lymphoma related factor, plays a crucial role in cell differentiation and is characterized by its unique structure, which includes a poxvirus zinc finger (POZ) domain at the N-terminus and Kruppel-type zinc fingers at the C-terminus. This structural configuration facilitates protein-protein interactions essential for LRF′s function as a transcription factor. LRF influences the transition from cellular proliferation to terminal differentiation, particularly during the differentiation of preadipocytes, thereby contributing to adipogenesis. Additionally, LRF can associate with active chromatin and enhance HIV-1 transcription by interacting with the long terminal repeat, highlighting LRF′s importance in both normal cellular processes and viral pathogenesis. LRF′s ability to modulate the expression of key cell cycle regulators, such as cyclin A and cyclin-dependent kinase 2, further underscores LRF′s role in controlling cell growth and differentiation. With versatile applications and critical biological functions, anti-LRF antibody (H-6) serves as an invaluable tool for researchers studying cellular differentiation and related pathways.

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LRF/Pokemon Antibody (H-6) References:

1. LRF is an essential downstream target of GATA1 in erythroid development and regulates BIM-dependent apoptosis.  |  Maeda, T., et al. 2009. Dev Cell. 17: 527-40. PMID: 19853566
2. LRF-mediated Dll4 repression in erythroblasts is necessary for hematopoietic stem cell maintenance.  |  Lee, SU., et al. 2013. Blood. 121: 918-29. PMID: 23134786
3. Attenuation of Leukemia/Lymphoma-Related Factor Protein Expression Inhibits Glioma Cell Proliferation and Invasion.  |  Huang, R., et al. 2015. J Environ Pathol Toxicol Oncol. 34: 125-31. PMID: 26081031
4. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair.  |  Liu, XS., et al. 2015. Nat Commun. 6: 8325. PMID: 26446488
5. Leukemia/lymphoma-related factor (LRF) exhibits stage- and context-dependent transcriptional controls in the oligodendrocyte lineage and modulates remyelination.  |  Davidson, NL., et al. 2017. J Neurosci Res. 95: 2391-2408. PMID: 28556945
6. LRF inhibits p53 expression in colon cancer cells via modulating DAP5 activity.  |  Zhu, M., et al. 2017. Cell Biochem Funct. 35: 401-406. PMID: 28849590
7. The transcription factor LRF promotes integrin β7 expression by and gut homing of CD8αα+ intraepithelial lymphocyte precursors.  |  Nie, J., et al. 2022. Nat Immunol. 23: 594-604. PMID: 35354951
8. Leukemia/lymphoma-related factor (LRF) or osteoclast zinc finger protein (OCZF) overexpression promotes osteoclast survival by increasing Bcl-xl mRNA: A novel regulatory mechanism mediated by the RNA binding protein SAM68.  |  Xu, X., et al. 2022. Lab Invest. 102: 1000-1010. PMID: 35474350
9. LRF Promotes Indirectly Advantageous Chromatin Conformation via BGLT3-lncRNA Expression and Switch from Fetal to Adult Hemoglobin.  |  Chondrou, V., et al. 2022. Int J Mol Sci. 23: PMID: 35806029
10. Transcriptional repression of lncRNA and miRNA subsets mediated by LRF during erythropoiesis.  |  Athanasopoulou, K., et al. 2023. J Mol Med (Berl). 101: 1097-1112. PMID: 37486375

The preferred LRF/Pokemon antibody is LRF/Pokemon Antibody (13E9): sc-33683.