LRCH4 Double Nickase Plasmid (h): sc-405444-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRCH4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRCH4 Double Nickase Plasmid (h) and LRCH4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LRCH4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRCH4 Antibody (A-9): sc-377441
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRCH4 Double Nickase Plasmid (h) | sc-405444-NIC | 20 µg | $410.00 | |||
LRCH4 Double Nickase Plasmid (h2) | sc-405444-NIC-2 | 20 µg | $410.00 |
LRCH4 (leucine-rich repeats and calponin homology domain containing 4) encodes a cytosolic protein characterized by LRR and CH-like domains that are commonly associated with protein–protein interactions and cytoskeletal organization. Emerging evidence links LRCH family members to regulation of actin dynamics, membrane trafficking, and innate immune signaling, with LRCH4 implicated in modulation of receptor-proximal inflammatory pathways such as TLR-mediated responses. Through these processes, LRCH4 may influence cell motility, adhesion, and stress-responsive transcriptional programs in immune and epithelial contexts. Dysregulated inflammatory signaling and cytoskeletal remodeling are recurrent features across immune-mediated disorders and cancer biology, making LRCH4 a useful target for mechanistic pathway studies.
LRCH4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LRCH4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LRCH4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LRCH4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LRCH4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.