Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LOX Double Nickase Plasmid (h): sc-400973-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOX Double Nickase Plasmid (h) and LOX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LOX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOX Antibody (F-8): sc-373995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOX Double Nickase Plasmid (h) | sc-400973-NIC | 20 µg | $410.00 | |||
LOX Double Nickase Plasmid (h2) | sc-400973-NIC-2 | 20 µg | $410.00 |
LOX encodes lysyl oxidase, a secreted copper-dependent amine oxidase that catalyzes oxidative deamination of lysine residues in collagen and elastin, initiating covalent crosslinking that stabilizes extracellular matrix architecture. Through regulation of matrix stiffness and collagen fibrillogenesis, LOX influences cell adhesion, migration, and mechanotransduction pathways, including integrin/FAK signaling and ECM remodeling programs. Altered LOX activity is linked to fibrosis and aberrant connective tissue remodeling, and its dysregulation has been associated with changes in tumor microenvironment dynamics and metastatic niche formation in multiple cancers. As a matrix-modifying enzyme, LOX is frequently studied in contexts of wound repair, vascular biology, and tissue mechanics.
LOX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LOX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LOX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LOX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LOX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.