LOH12CR1 CRISPR/Cas9 KO Plasmid (m2): sc-426735-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOH12CR1 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOH12CR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOH12CR1 CRISPR/Cas9 KO Plasmid (m2) | sc-426735-KO-2 | 20 µg | $397.00 |
Overview
Borcs5 encodes LOH12CR1, a component of the BORC (BLOC-1–related complex) that supports peripheral positioning of lysosomes and lysosome-related organelles by coupling them to microtubule-based transport. Through this role, LOH12CR1 contributes to endolysosomal trafficking, spatial control of degradative capacity, and processes such as autophagy and membrane protein turnover that depend on lysosome distribution. BORC-dependent lysosome dispersal interfaces with small GTPase and kinesin-driven pathways that coordinate organelle motility and cellular homeostasis. Dysregulation of lysosome positioning and trafficking is broadly relevant to neurobiology and to experimental models of neurodegeneration and inflammation where altered autophagic flux and organelle transport are common phenotypes.
LOH12CR1 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Borcs5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Borcs5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Borcs5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOH12CR1 protein expression.
This CRISPR knockout system enables efficient generation of Borcs5-deficient cell models for investigation of LOH12CR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Borcs5 exon(s) critical for LOH12CR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Borcs5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOH12CR1 CRISPR/Cas9 KO Plasmid (m) and LOH12CR1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Borcs5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOH12CR1 HDR Plasmid (m) and LOH12CR1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Borcs5 homology arms to support homology-directed repair at defined Borcs5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.