LOC729974 CRISPR/Cas9 KO Plasmid (h2): sc-418913-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC729974 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOC729974 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC729974 CRISPR/Cas9 KO Plasmid (h2) | sc-418913-KO-2 | 20 µg | $397.00 |
Overview
RFPL4AL1 encodes LOC729974, a putative member of the ret finger protein–like family that is predicted to participate in protein–protein interaction networks and ubiquitin-associated regulation of cellular homeostasis. Although functional annotation remains limited, RFPL-like proteins are commonly linked to control of protein stability, transcriptional programs, and cell-cycle–associated processes that shape proliferation and differentiation states. This gene is therefore of interest for dissecting pathways governing proteostasis and regulatory signaling in human cells, particularly where aberrant turnover of key regulators contributes to disease-relevant phenotypes. Loss-of-function interrogation can help clarify whether LOC729974 influences stress responses, chromatin-associated regulation, or other context-specific cellular programs.
LOC729974 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the RFPL4AL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RFPL4AL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RFPL4AL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOC729974 protein expression.
This CRISPR knockout system enables efficient generation of RFPL4AL1-deficient cell models for investigation of LOC729974 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RFPL4AL1 exon(s) critical for LOC729974 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RFPL4AL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOC729974 CRISPR/Cas9 KO Plasmid (h) and LOC729974 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RFPL4AL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOC729974 HDR Plasmid (h) and LOC729974 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RFPL4AL1 homology arms to support homology-directed repair at defined RFPL4AL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.