Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LMP2 Double Nickase Plasmid (h): sc-401615-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LMP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LMP2 Double Nickase Plasmid (h) and LMP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PSMB9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LMP2 Antibody (G-3): sc-373996
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LMP2 Double Nickase Plasmid (h) | sc-401615-NIC | 20 µg | $410.00 | |||
LMP2 Double Nickase Plasmid (h2) | sc-401615-NIC-2 | 20 µg | $410.00 |
PSMB9 encodes the human immunoproteasome catalytic subunit LMP2 (β1i), which replaces the constitutive β1 subunit in response to interferon-γ and other inflammatory cues. LMP2 contributes to ubiquitin-dependent proteolysis and shapes the peptide repertoire generated for MHC class I antigen processing and presentation, thereby influencing CD8+ T cell recognition. Through its role in immunoproteasome assembly and proteasomal cleavage preferences, PSMB9 links innate inflammatory signaling to adaptive immune surveillance and cellular protein homeostasis. Altered PSMB9 activity or expression has been associated with dysregulated antigen presentation and inflammatory phenotypes relevant to autoimmunity, infection biology, and tumor immune interactions.
LMP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PSMB9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PSMB9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PSMB9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PSMB9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.