LMO4 Double Nickase Plasmid (m): sc-421448-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LMO4 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LMO4 Double Nickase Plasmid (m) and LMO4 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lmo4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LMO4 Antibody (4H8): sc-293440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LMO4 Double Nickase Plasmid (m) | sc-421448-NIC | 20 µg | $410.00 | |||
LMO4 Double Nickase Plasmid (m2) | sc-421448-NIC-2 | 20 µg | $410.00 |
Lmo4 encodes LIM domain only 4 (LMO4), a nuclear LIM-only adaptor protein that assembles multi-protein transcriptional complexes and modulates context-dependent gene expression programs. In mouse tissues, LMO4 contributes to developmental patterning and cell fate decisions by coordinating interactions among LIM-binding partners and other transcriptional regulators, influencing processes such as differentiation, proliferation, and neuronal function. LMO4 has been linked to pathways controlling transcriptional regulation and signal integration, including networks implicated in epithelial and neural development. Dysregulated LMO4 activity has been associated with aberrant transcriptional control observed in models of tumorigenesis and neurodevelopmental phenotypes, supporting its use as a mechanistic node in gene regulatory studies.
LMO4 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lmo4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lmo4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lmo4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lmo4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.