LL-37 Antibody (D-5): sc-166770

LL-37 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the LL-37 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-LL-37 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LL-37, a member of the cathelicidin family, plays a crucial role in the innate immune response, acting as an antimicrobial peptide that helps prevent infections by directly disrupting microbial membranes. A unique structure, characterized by an amphipathic α-helical conformation, allows LL-37 to interact effectively with bacterial lipopolysaccharides, enhancing the ability to recruit immune cells such as mast cells to sites of inflammation. This recruitment is vital for initiating a robust immune response, particularly in inflammatory skin conditions like psoriasis and dermatitis, where LL-37 levels are significantly elevated. LL-37 is primarily expressed in bone marrow, testis, and neutrophils, and an absence in normal skin epidermis highlights a role in pathological conditions. Additionally, the mouse and rat ortholog, CRAMP (cathelin-related antimicrobial peptide), shares similar antimicrobial properties and is expressed in various tissues, including the testis and intestine, further underscoring the importance of cathelicidins in host defense mechanisms.

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LL-37 Antibody (D-5) References:

1. Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3.  |  Sørensen, OE., et al. 2001. Blood. 97: 3951-9. PMID: 11389039
2. A cathelicidin family of human antibacterial peptide LL-37 induces mast cell chemotaxis.  |  Niyonsaba, F., et al. 2002. Immunology. 106: 20-6. PMID: 11972628
3. Cathelicidin antimicrobial peptides are expressed in salivary glands and saliva.  |  Murakami, M., et al. 2002. J Dent Res. 81: 845-50. PMID: 12454100
4. Neonatal skin in mice and humans expresses increased levels of antimicrobial peptides: innate immunity during development of the adaptive response.  |  Dorschner, RA., et al. 2003. Pediatr Res. 53: 566-72. PMID: 12612195
5. Phylogeny, processing and expression of the rat cathelicidin rCRAMP: a model for innate antimicrobial peptides.  |  Termén, S., et al. 2003. Cell Mol Life Sci. 60: 536-49. PMID: 12737313
6. The cathelicidins--structure, function and evolution.  |  Tomasinsig, L. and Zanetti, M. 2005. Curr Protein Pept Sci. 6: 23-34. PMID: 15638766
7. LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity.  |  Wei, X., et al. 2022. Cell Rep. 39: 110880. PMID: 35649354
8. LL-37, a Multi-Faceted Amphipathic Peptide Involved in NETosis.  |  Radic, M. and Muller, S. 2022. Cells. 11: PMID: 35954305
9. Regulation of LL-37 in Bone and Periodontium Regeneration.  |  Chinipardaz, Z., et al. 2022. Life (Basel). 12: PMID: 36294968
10. A novel murine cathelin-like protein expressed in bone marrow.  |  Popsueva, AE., et al. 1996. FEBS Lett. 391: 5-8. PMID: 8706928
11. Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse.  |  Gallo, RL., et al. 1997. J Biol Chem. 272: 13088-93. PMID: 9148921
12. The expression of the gene coding for the antibacterial peptide LL-37 is induced in human keratinocytes during inflammatory disorders.  |  Frohm, M., et al. 1997. J Biol Chem. 272: 15258-63. PMID: 9182550

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LL-37 Antibody (D-5) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-LL-37 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LL-37 Antibody (D-5) Specifications

Alternate Product Name: cathelicidin antimicrobial peptide (CAMP)

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 131-170

Epitope Target: LL-37 proteolytic fragment of CAP-18

Epitope Species: human

Product Reactivity/Detection: mature LL-37

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for LL-37 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin