Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LKB1 Double Nickase Plasmid (m): sc-423192-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LKB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LKB1 Double Nickase Plasmid (m) and LKB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Stk11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LKB1 Antibody (Ley 37D/G6): sc-32245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LKB1 Double Nickase Plasmid (m) | sc-423192-NIC | 20 µg | $410.00 | |||
LKB1 Double Nickase Plasmid (m2) | sc-423192-NIC-2 | 20 µg | $410.00 |
Mouse Stk11 encodes the serine/threonine kinase LKB1, a master regulator of cellular energy homeostasis and polarity that activates AMPK and a broad set of AMPK-related kinases. Through this signaling axis, LKB1 coordinates metabolic stress responses, mitochondrial function, autophagy, and cell-cycle control, and it contributes to maintenance of epithelial architecture via polarity complexes. Disruption of LKB1-dependent pathways is associated with altered growth control and metabolic reprogramming, making Stk11 a key node in studies of tumor suppressor biology. In mouse systems, LKB1 perturbation is widely used to interrogate tissue-specific regulation of metabolism, stem cell behavior, and microenvironmental stress adaptation.
LKB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Stk11 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Stk11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Stk11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Stk11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.