Lingo-1 CRISPR/Cas9 KO Plasmid (h): sc-404453
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lingo-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Lingo-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lingo-1 CRISPR/Cas9 KO Plasmid (h) | sc-404453 | 20 µg | $397.00 |
Overview
LINGO1 encodes Lingo-1, a CNS-enriched leucine-rich repeat and immunoglobulin domain-containing transmembrane protein that functions as a component of receptor complexes regulating neuronal growth and myelination. Lingo-1 interacts with Nogo receptor signaling and related pathways to modulate axon guidance, neurite outgrowth, oligodendrocyte differentiation, and cytoskeletal remodeling. Through effects on neuron–glia communication and inhibitory cues in the nervous system, LINGO1 has been studied in the context of demyelination, axonal regeneration failure, and neurodegeneration-associated processes. Altered LINGO1 expression or signaling has also been explored for its potential impact on synaptic plasticity and neuroinflammatory responses in experimental models.
Lingo-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LINGO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LINGO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LINGO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Lingo-1 protein expression.
This CRISPR knockout system enables efficient generation of LINGO1-deficient cell models for investigation of Lingo-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LINGO1 exon(s) critical for Lingo-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LINGO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Lingo-1 CRISPR/Cas9 KO Plasmid (h) and Lingo-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LINGO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Lingo-1 HDR Plasmid (h) and Lingo-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LINGO1 homology arms to support homology-directed repair at defined LINGO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.