LIMP II Antibody (D-4): sc-55571

LIMP II Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects LIMP II protein in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LIMP II (D-4) antibody is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants. LIMP II, a lysosomal integral membrane protein family member, maintains proper lysosomal function for cellular waste disposal and recycling. LIMP II (D-4) monoclonal antibody helps researchers study lysosomal membrane dynamics, where LIMP II facilitates lysosome fusion with phagocytic vacuoles for material degradation. Anti-LIMP II antibody (D-4) serves as a valuable research tool for investigating protein trafficking to lysosomes, cellular debris processing, and overall lysosomal integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LIMP II Antibody (D-4) References:

1. Bi-directional trafficking between the trans-Golgi network and the endosomal/lysosomal system.  |  Rohn, WM., et al. 2000. J Cell Sci. 113 (Pt 12): 2093-101. PMID: 10825282
2. Targeting of lysosomal integral membrane protein LIMP II. The tyrosine-lacking carboxyl cytoplasmic tail of LIMP II is sufficient for direct targeting to lysosomes.  |  Vega, MA., et al. 1991. J Biol Chem. 266: 16269-72. PMID: 1715864
3. Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease.  |  Peters, J., et al. 2015. Biochem Biophys Res Commun. 457: 334-40. PMID: 25576872
4. Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export.  |  Heybrock, S., et al. 2019. Nat Commun. 10: 3521. PMID: 31387993
5. LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma.  |  Liu, Y., et al. 2023. Int J Oral Sci. 15: 24. PMID: 37291150
6. Uridine-Modified Ruthenium(II) Complex as Lysosomal LIMP-2 Targeting Photodynamic Therapy Photosensitizer for the Treatment of Triple-Negative Breast Cancer.  |  Wu, Q., et al. 2024. JACS Au. 4: 1081-1096. PMID: 38559730
7. Activation and Purification of ß-Glucocerebrosidase by Exploiting its Transporter LIMP-2 - Implications for Novel Treatment Strategies in Gaucher's and Parkinson's Disease.  |  Dobert, JP., et al. 2024. Adv Sci (Weinh). 11: e2401641. PMID: 38666485
8. The lysosomal lipid transporter LIMP-2 is part of lysosome-ER STARD3-VAPB-dependent contact sites.  |  Rudnik, S., et al. 2024. J Cell Sci. 137: PMID: 39370902
9. The lysosomal proenzyme receptor that binds procathepsin L to microsomal membranes at pH 5 is a 43-kDa integral membrane protein.  |  McIntyre, GF. and Erickson, AH. 1993. Proc Natl Acad Sci U S A. 90: 10588-92. PMID: 8248149
10. The tyrosine-based lysosomal targeting signal in lamp-1 mediates sorting into Golgi-derived clathrin-coated vesicles.  |  Höning, S., et al. 1996. EMBO J. 15: 5230-9. PMID: 8895568
11. Lysosomal integral membrane protein II binds thrombospondin-1. Structure-function homology with the cell adhesion molecule CD36 defines a conserved recognition motif.  |  Crombie, R. and Silverstein, R. 1998. J Biol Chem. 273: 4855-63. PMID: 9478926
12. The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins.  |  Le Borgne, R., et al. 1998. J Biol Chem. 273: 29451-61. PMID: 9792650

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LIMP II Antibody (D-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-LIMP II Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LIMP II Antibody (D-4) Specifications

Alternate Product Name: LIMP II

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 249-478

Epitope Target: LIMP II

Epitope Species: human

Product Reactivity/Detection: LIMP II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for LIMP II Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LIMP II antibody is LIMP II Antibody (D-3): sc-55570.