RNAi-directed mRNA Cleavage
LIME siRNA and shRNA Plasmids (m)
- Target species: mouse
- Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
- For independent verification of LIME gene silencing results, individual duplex components or plasmids are also available upon request
- Suitable control antibody: LIME Antibody (A-7): sc-365195 is recommended as control antibody for monitoring of LIME expression knockdown by Western blotting or immunofluorescence
- Get details about si/shRNA Gene Silencers
- Gene silencers are intended for noncommercial and research use only. Click here for details.
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siRNA
Primer
shRNA Plasmid
shRNA Lentiviral Particles
Recommended Control Antibody
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LIME Antibodies for analysis of cellular responses to LIME si/shRNA Gene Silencers
Description
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of LIME gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: LIME Antibody (A-7): sc-365195
- sqRT-PCR Primer also available: LIME (m)-PR: sc-60935-PR, 20 µl
- Get details about si/shRNA Gene Silencers
See Protocol Online.
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Description
- 20 µl of each primer set is provided at a concentration of 10 µM
- LIME Primer (m) includes A set (forward & reverse) and B set (forward & reverse) primers
- Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR
See Protocol Online.
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Description
- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to LIME siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use.
- suitable control antibody: LIME Antibody (A-7): sc-365195
- sqRT-PCR Primer also available: LIME (m)-PR: sc-60935-PR, 20 µl
See Protocol Online.
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Description
- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to LIME siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
- Lentiviral Particles are intended for non-commercial use.
See Protocol Online.
DOWNLOADS
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Related Products
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LIME siRNA (m) | sc-60935 | 10 µM | $298.00 | |||
LIME (m)-PR | sc-60935-PR | 10 µM, 20 µl | $34.00 | |||
LIME shRNA Plasmid (m) | sc-60935-SH | 20 µg | $603.00 | |||
LIME shRNA (m) Lentiviral Particles | sc-60935-V | 200 µl | $725.00 |