Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Limd1 CRISPR/Cas9 KO Plasmid (m): sc-424343
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Limd1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Limd1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Limd1 Antibody (H-4): sc-271448
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Limd1 CRISPR/Cas9 KO Plasmid (m) | sc-424343 | 20 µg | $397.00 |
Overview
LIM domain containing 1 (Limd1) is a scaffolding protein that localizes to focal adhesions and cell–cell junctions, coordinating protein–protein interactions that shape cytoskeletal organization and mechanotransduction. Limd1 participates in pathways governing cell adhesion, migration, and growth control, and has been linked to regulation of Hippo/YAP signaling through interactions that influence LATS activity and YAP/TAZ localization. In mouse systems, Limd1 supports studies of tissue homeostasis and developmental programs where adhesion-dependent signaling is critical. Dysregulation of LIMD1-associated networks is relevant to tumor suppressor biology and metastasis-associated phenotypes, including altered invasion and epithelial–mesenchymal transition–like processes.
Limd1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Limd1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Limd1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Limd1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Limd1 protein expression.
This CRISPR knockout system enables efficient generation of Limd1-deficient cell models for investigation of Limd1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Limd1 exon(s) critical for Limd1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Limd1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Limd1 CRISPR/Cas9 KO Plasmid (m) and Limd1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Limd1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Limd1 HDR Plasmid (m) and Limd1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Limd1 homology arms to support homology-directed repair at defined Limd1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.