LGR5 Antibody (634J2E): sc-517661

LGR5 Antibody (634J2E) is a mouse monoclonal IgG1 antibody that detects the LGR5 protein of human origin by western blotting (WB) and flow cytometry (FCM). LGR5, or leucine-rich repeat-containing G-protein coupled receptor 5, is a 907 amino acid multi-pass membrane protein characterized by 17 leucine-rich repeats, which are crucial for structural integrity and function as a G protein-coupled receptor. LGR5 protein is predominantly expressed in placenta, skeletal muscle, and spinal cord, where LGR5 plays a vital role as an orphan receptor involved in embryonic growth control and cellular differentiation. The significance of LGR5 extends beyond normal physiological processes; LGR5 overexpression has been linked to increased tumor susceptibility and malignant transformation, highlighting LGR5′s potential as a key player in cancer biology. Anti-LGR5 antibody (634J2E) enables exploration of LGR5 mechanisms in both normal development and pathological conditions, providing valuable insights into LGR5′s role in tumorigenesis and cellular signaling pathways.

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LGR5 Antibody (634J2E) References:

1. Identification of stem cells in small intestine and colon by marker gene Lgr5.  |  Barker, N., et al. 2007. Nature. 449: 1003-7. PMID: 17934449
2. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.  |  Sato, T., et al. 2009. Nature. 459: 262-5. PMID: 19329995
3. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.  |  Snippert, HJ., et al. 2010. Cell. 143: 134-44. PMID: 20887898
4. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts.  |  Sato, T., et al. 2011. Nature. 469: 415-8. PMID: 21113151
5. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.  |  Huch, M., et al. 2013. Nature. 494: 247-50. PMID: 23354049
6. The R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength.  |  de Lau, W., et al. 2014. Genes Dev. 28: 305-16. PMID: 24532711
7. Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer.  |  Fumagalli, A., et al. 2020. Cell Stem Cell. 26: 569-578.e7. PMID: 32169167
8. LGR5 expressing skin fibroblasts define a major cellular hub perturbed in scleroderma.  |  Gur, C., et al. 2022. Cell. 185: 1373-1388.e20. PMID: 35381199
9. Functional patient-derived organoid screenings identify MCLA-158 as a therapeutic EGFR × LGR5 bispecific antibody with efficacy in epithelial tumors.  |  Herpers, B., et al. 2022. Nat Cancer. 3: 418-436. PMID: 35469014
10. Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity.  |  Ruscitto, A., et al. 2023. Cell Stem Cell. 30: 1179-1198.e7. PMID: 37683603