LGA Antibody (175I2M): sc-517626
- LGA Antibody (175I2M) is a mouse monoclonal IgG1 κ LGA antibody provided at 100 µg/ml
- raised against recombinant LGA of human origin
- recommended for detection of LGA of mouse, rat and human origin by WB, IF and IHC(P)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LGA Antibody (175I2M). This work is in progress.
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LGA Antibody (175I2M) is a mouse monoclonal IgG1 kappa light chain antibody that detects LGA protein of mouse, rat, and human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-LGA antibody (175I2M) is available as a non-conjugated format. LGA protein, also known as L-glutamine amidohydrolase or GLS2 (glutaminase 2, liver, mitochondrial), is a 602 amino acid protein primarily localized in the mitochondrion and features two ankyrin repeats, which are important for protein-protein interactions. LGA functions as a mitochondrial phosphate-activated glutaminase, catalyzing the conversion of glutamine to glutamate and ammonia, a process vital for cellular metabolism and energy production. LGA is overexpressed in breast cancer cell lines, indicating potential involvement in tumorigenesis and making LGA a significant target for cancer research. LGA gene is located on human chromosome 12, a region associated with various diseases, including hypochondrogenesis and Noonan syndrome, highlighting the importance of studying LGA in the context of both normal physiology and disease.
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LGA Antibody (175I2M) References:
- Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. | Gómez-Fabre, PM., et al. 2000. Biochem J. 345 Pt 2: 365-75. PMID: 10620514
- Identification of two human glutaminase loci and tissue-specific expression of the two related genes. | Aledo, JC., et al. 2000. Mamm Genome. 11: 1107-10. PMID: 11130979
- The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins. | Olalla, L., et al. 2001. FEBS Lett. 488: 116-22. PMID: 11163757
- Nuclear localization of L-type glutaminase in mammalian brain. | Olalla, L., et al. 2002. J Biol Chem. 277: 38939-44. PMID: 12163477
- Genomic organization and transcriptional analysis of the human l-glutaminase gene. | Pérez-Gómez, C., et al. 2003. Biochem J. 370: 771-84. PMID: 12444921
- Mitochondrial glutaminase enhances extracellular glutamate production in HIV-1-infected macrophages: linkage to HIV-1 associated dementia. | Zhao, J., et al. 2004. J Neurochem. 88: 169-80. PMID: 14675161
- Co-expression of glutaminase K and L isoenzymes in human tumour cells. | Pérez-Gómez, C., et al. 2005. Biochem J. 386: 535-42. PMID: 15496140
- HIV-infected macrophages mediate neuronal apoptosis through mitochondrial glutaminase. | Tian, C., et al. 2008. J Neurochem. 105: 994-1005. PMID: 18088378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LGA Antibody (175I2M) | sc-517626 | 100 µg/ml | $322.00 |