Lex A Antibody (2-12): sc-7544

Lex A Antibody (2-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Lex A in various applications including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Lex A antibody (2-12) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Lex A, a crucial protein involved in the regulation of gene expression, plays a significant role in the two-hybrid system, a powerful method for studying protein-protein interactions. This system utilizes genetically engineered yeast or bacteria, where a ′bait′ protein is fused to a DNA-binding domain (DBD) and a ′prey′ protein is linked to a transcriptional activation domain (AD). The interaction between these proteins activates reporter genes, allowing researchers to identify and analyze protein interactions effectively. The adaptability of the two-hybrid system, which incorporates DBDs like GAL4 or LexA and ADs from GAL4 or the herpes simplex virus protein VP16, has made this approach a preferred choice for proteomics studies and interactome mapping. The ability to utilize prokaryotic proteins, such as Escherichia coli LexA′s DBD, alongside the N-terminal of GAL4 from Saccharomyces cerevisiae, enhances experimental versatility across various organisms, ensuring specific transcriptional activation and appropriate localization of proteins within the cell.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Lex A Antibody (2-12) References:

1. GAL4 fusion vectors for expression in yeast or mammalian cells.  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
2. The bacterial LexA transcriptional repressor.  |  Butala, M., et al. 2009. Cell Mol Life Sci. 66: 82-93. PMID: 18726173
3. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
4. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
5. Activators and targets.  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
6. A novel genetic system to detect protein-protein interactions.  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
7. The Use and Abuse of LexA by Mobile Genetic Elements.  |  Fornelos, N., et al. 2016. Trends Microbiol. 24: 391-401. PMID: 26970840
8. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
9. Deletion analysis of GAL4 defines two transcriptional activating segments.  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
10. Nanobodies targeting LexA autocleavage disclose a novel suppression strategy of SOS-response pathway.  |  Maso, L., et al. 2022. Structure. 30: 1479-1493.e9. PMID: 36240773
11. Purified lexA protein is a repressor of the recA and lexA genes.  |  Little, JW., et al. 1981. Proc Natl Acad Sci U S A. 78: 4199-203. PMID: 7027255
12. The two-hybrid system: an assay for protein-protein interactions.  |  Fields, S. and Sternglanz, R. 1994. Trends Genet. 10: 286-92. PMID: 7940758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lex A Antibody (2-12) has 79 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Lex A Antibody (2-12) Specifications

Alternate Product Name: Lex A

Product Clone Name: 2-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Lex A

Epitope Species:

Product Reactivity/Detection: Lex A and Lex A fusion proteins

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 79 citations in various scientific publications

Available Conjugates/Forms for Lex A Antibody (2-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin