leupaxin Double Nickase Plasmid (h): sc-405101-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- leupaxin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- leupaxin Double Nickase Plasmid (h) and leupaxin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LPXN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: leupaxin Antibody (B-2): sc-376820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
leupaxin Double Nickase Plasmid (h) | sc-405101-NIC | 20 µg | $410.00 | |||
leupaxin Double Nickase Plasmid (h2) | sc-405101-NIC-2 | 20 µg | $410.00 |
Human LPXN encodes leupaxin, a paxillin family LIM domain adaptor that localizes to focal adhesions and links integrin engagement to downstream signaling. Through interactions with cytoskeletal and tyrosine kinase complexes, leupaxin modulates cell adhesion, migration, and mechanotransduction, influencing focal adhesion turnover and actin dynamics. LPXN-dependent signaling interfaces with pathways such as FAK/SRC, Rho-family GTPase regulation, and MAPK cascades that coordinate cell motility and survival cues. Altered leupaxin expression or focal-adhesion rewiring has been associated with invasive cellular phenotypes in cancer biology and with dysregulated immune cell trafficking, making LPXN a useful node for studying adhesion-linked disease mechanisms.
leupaxin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LPXN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LPXN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LPXN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LPXN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.