leupaxin Antibody (B-2): sc-376820

leupaxin Antibody (B-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects leupaxin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). leupaxin Antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. leupaxin, also known as LDPL or LPXN, is a 386 amino acid cytoplasmic protein that belongs to the paxillin family and plays a crucial role in cellular signaling. leupaxin is predominantly expressed in lymphoid tissues such as the spleen, lymph nodes, thymus, and appendix, while exhibiting lower levels in bone marrow and fetal liver. leupaxin features four leucine-rich LD-motifs at its N-terminus and four LIM domains at its C-terminus, which are essential for interaction with focal adhesion kinase family members, particularly PYK2, in lymphoid cells. This interaction is vital for regulating cell type-specific signaling at adhesion sites, highlighting leupaxin′s importance in cellular communication and response. Additionally, leupaxin serves as a substrate for tyrosine kinases in lymphoid cells, suggesting involvement in tyrosine kinase-mediated signaling pathways. Notably, leupaxin has been identified as a potential progression marker for a subset of prostate cancer and may function as a novel coactivator of the androgen receptor, underscoring its significance in cancer biology and therapeutic targeting.

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leupaxin Antibody (B-2) References:

1. Leupaxin is a critical adaptor protein in the adhesion zone of the osteoclast.  |  Gupta, A., et al. 2003. J Bone Miner Res. 18: 669-85. PMID: 12674328
2. Leupaxin binds to PEST domain tyrosine phosphatase PEP.  |  Watanabe, N., et al. 2005. Mol Cell Biochem. 269: 13-7. PMID: 15786712
3. Leupaxin negatively regulates B cell receptor signaling.  |  Chew, V. and Lam, KP. 2007. J Biol Chem. 282: 27181-27191. PMID: 17640867
4. Leupaxin, a novel coactivator of the androgen receptor, is expressed in prostate cancer and plays a role in adhesion and invasion of prostate carcinoma cells.  |  Kaulfuss, S., et al. 2008. Mol Endocrinol. 22: 1606-21. PMID: 18451096
5. The LIM protein leupaxin is enriched in smooth muscle and functions as an serum response factor cofactor to induce smooth muscle cell gene transcription.  |  Sundberg-Smith, LJ., et al. 2008. Circ Res. 102: 1502-11. PMID: 18497331
6. Leupaxin acts as a mediator in prostate carcinoma progression through deregulation of p120catenin expression.  |  Kaulfuss, S., et al. 2009. Oncogene. 28: 3971-82. PMID: 19701244
7. LIM domain-containing adaptor, leupaxin, localizes in focal adhesion and suppresses the integrin-induced tyrosine phosphorylation of paxillin.  |  Tanaka, T., et al. 2010. Cancer Sci. 101: 363-8. PMID: 19917054
8. Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading.  |  Chen, PW. and Kroog, GS. 2010. Cell Adh Migr. 4: 527-40. PMID: 20543562
9. Leupaxin is expressed in mammary carcinoma and acts as a transcriptional activator of the estrogen receptor α.  |  Kaulfuss, S., et al. 2015. Int J Oncol. 47: 106-14. PMID: 25955236
10. A kindlin-3-leupaxin-paxillin signaling pathway regulates podosome stability.  |  Klapproth, S., et al. 2019. J Cell Biol. 218: 3436-3454. PMID: 31537712
11. Leupaxin Expression Is Dispensable for B Cell Immune Responses.  |  Bonaud, A., et al. 2020. Front Immunol. 11: 466. PMID: 32269569
12. Leupaxin is a novel LIM domain protein that forms a complex with PYK2.  |  Lipsky, BP., et al. 1998. J Biol Chem. 273: 11709-13. PMID: 9565592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-leupaxin Antibody (B-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-leupaxin Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

leupaxin Antibody (B-2) Specifications

Alternate Product Name: leupaxin

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 13-45

Epitope Target: leupaxin

Epitope Species: human

Product Reactivity/Detection: leupaxin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for leupaxin Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin