LENG4 CRISPR/Cas9 KO Plasmid (h): sc-408536
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LENG4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LENG4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LENG4 CRISPR/Cas9 KO Plasmid (h) | sc-408536 | 20 µg | $397.00 |
Overview
MBOAT7 encodes an endoplasmic reticulum–localized membrane-bound O-acyltransferase that remodels phosphatidylinositol by incorporating arachidonoyl-CoA, thereby shaping cellular phosphoinositide composition and downstream signaling. Through effects on membrane lipid homeostasis, MBOAT7 influences PI-derived signaling nodes that regulate vesicular trafficking, cytoskeletal dynamics, and inflammatory responses. Altered MBOAT7 activity or expression has been linked to metabolic and hepatic phenotypes, including susceptibility to lipid dysregulation and liver injury, making it relevant for studies of immunometabolism and stress signaling. LENG4 (leukocyte receptor cluster member 4) is a human protein with emerging roles in immune-associated contexts, supporting pathway-level interrogation of lipid signaling–immune crosstalk in relevant cell models.
LENG4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MBOAT7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MBOAT7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MBOAT7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LENG4 protein expression.
This CRISPR knockout system enables efficient generation of MBOAT7-deficient cell models for investigation of LENG4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MBOAT7 exon(s) critical for LENG4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MBOAT7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LENG4 CRISPR/Cas9 KO Plasmid (h) and LENG4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MBOAT7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LENG4 HDR Plasmid (h) and LENG4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MBOAT7 homology arms to support homology-directed repair at defined MBOAT7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.