LCRG1 CRISPR/Cas9 KO Plasmid (h): sc-410137
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LCRG1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LCRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LCRG1 CRISPR/Cas9 KO Plasmid (h) | sc-410137 | 20 µg | $397.00 |
Overview
GGNBP2 (also known as LCRG1) encodes a nuclear protein implicated in transcriptional regulation and maintenance of cellular growth control programs. Reported functions link LCRG1 to modulation of cell-cycle progression, chromatin-associated processes, and context-dependent regulation of proliferation and differentiation pathways. Altered GGNBP2/LCRG1 expression has been observed across multiple cancer types, supporting its utility as a research target for studying oncogenic signaling, genome stability, and tumor-associated transcriptional networks. In human cell models, perturbation of GGNBP2 can help dissect pathway-level effects on proliferation, apoptosis, and lineage-specific gene expression programs.
LCRG1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GGNBP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GGNBP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GGNBP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LCRG1 protein expression.
This CRISPR knockout system enables efficient generation of GGNBP2-deficient cell models for investigation of LCRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GGNBP2 exon(s) critical for LCRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GGNBP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LCRG1 CRISPR/Cas9 KO Plasmid (h) and LCRG1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GGNBP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LCRG1 HDR Plasmid (h) and LCRG1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GGNBP2 homology arms to support homology-directed repair at defined GGNBP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.