Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Latrophilin-3 CRISPR/Cas9 KO Plasmid (h): sc-411703
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Latrophilin-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Latrophilin-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Latrophilin-3 Antibody (B-6): sc-393576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Latrophilin-3 CRISPR/Cas9 KO Plasmid (h) | sc-411703 | 20 µg | $397.00 |
Overview
ADGRL3 encodes Latrophilin-3, an adhesion G protein-coupled receptor enriched in the nervous system that functions at cell–cell contacts to regulate synapse development, neurite outgrowth, and circuit assembly. Through its extracellular adhesion domains and GPCR signaling capacity, Latrophilin-3 links extracellular ligands to intracellular pathways that influence cytoskeletal remodeling, vesicle trafficking, and neuronal connectivity. Genetic and functional studies implicate ADGRL3 in neurodevelopmental and neuropsychiatric phenotypes, supporting its utility as a molecular entry point for dissecting synaptic organization and network-level signaling. Experimental interrogation of ADGRL3 is therefore relevant for understanding how adhesion-GPCR signaling contributes to neuronal differentiation, excitability, and synaptic maintenance.
Latrophilin-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADGRL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADGRL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADGRL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Latrophilin-3 protein expression.
This CRISPR knockout system enables efficient generation of ADGRL3-deficient cell models for investigation of Latrophilin-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADGRL3 exon(s) critical for Latrophilin-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADGRL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Latrophilin-3 CRISPR/Cas9 KO Plasmid (h) and Latrophilin-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADGRL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Latrophilin-3 HDR Plasmid (h) and Latrophilin-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADGRL3 homology arms to support homology-directed repair at defined ADGRL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.