LAT2 Antibody (3F10): sc-293242

LAT2 Antibody (3F10) is a mouse monoclonal IgG2a kappa light chain antibody that detects LAT2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-LAT2 antibody (3F10) is available as a non-conjugated monoclonal isotype antibody. L-amino acid transporter protein-2 (LAT2) is a crucial non-glycosylated membrane protein that plays a significant role in the transport of neutral amino acids across cell membranes, particularly in renal epithelia and blood-tissue barriers. LAT2 is primarily expressed in the kidney, but is also found in lower levels in various tissues, including the placenta, brain, liver, spleen, skeletal muscle, heart, small intestine, and lung. LAT2′s importance lies in its ability to facilitate the basolateral efflux of cysteine, which is essential for maintaining amino acid homeostasis in the body. Studies have shown that LAT2 expression is vital for the reabsorption of cysteine, making LAT2 a potential target for therapeutic interventions aimed at modulating amino acid transport. Furthermore, LAT2 forms a heterodimer with CD98, which interacts with Integrin β1 in intestinal epithelial cells, indicating that LAT2 activity can be influenced by cell adhesion events through ligand binding to CD98 and ICAM-1. This highlights a novel regulatory mechanism that connects cellular interactions with amino acid transport, underscoring the functional significance of LAT2 in physiological processes.

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LAT2 Antibody (3F10) References:

1. Identification of a membrane protein, LAT-2, that Co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids.  |  Pineda, M., et al. 1999. J Biol Chem. 274: 19738-44. PMID: 10391915
2. Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity.  |  Segawa, H., et al. 1999. J Biol Chem. 274: 19745-51. PMID: 10391916
3. LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine.  |  Rossier, G., et al. 1999. J Biol Chem. 274: 34948-54. PMID: 10574970
4. Basolateral LAT-2 has a major role in the transepithelial flux of L-cystine in the renal proximal tubule cell line OK.  |  Fernández, E., et al. 2003. J Am Soc Nephrol. 14: 837-47. PMID: 12660317
5. CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia.  |  Liu, X., et al. 2003. J Biol Chem. 278: 23672-7. PMID: 12716892
6. Organ-specific overexpression of renal LAT2 and enhanced tubular L-DOPA uptake precede the onset of hypertension.  |  Pinho, MJ., et al. 2003. Hypertension. 42: 613-8. PMID: 12975385
7. Over-expression of renal LAT1 and LAT2 and enhanced L-DOPA uptake in SHR immortalized renal proximal tubular cells.  |  Pinho, MJ., et al. 2004. Kidney Int. 66: 216-26. PMID: 15200428
8. L-leucine transport in human breast cancer cells (MCF-7 and MDA-MB-231): kinetics, regulation by estrogen and molecular identity of the transporter.  |  Shennan, DB., et al. 2004. Biochim Biophys Acta. 1664: 206-16. PMID: 15328053
9. Cloning and gene silencing of LAT2, the L-3,4-dihydroxyphenylalanine (L-DOPA) transporter, in pig renal LLC-PK1 epithelial cells.  |  Soares-Da-Silva, P., et al. 2004. FASEB J. 18: 1489-98. PMID: 15466357
10. System L-amino acid transporters are differently expressed in rat astrocyte and C6 glioma cells.  |  Kim, DK., et al. 2004. Neurosci Res. 50: 437-46. PMID: 15567481
11. Pharmacokinetic role of L-type amino acid transporters LAT1 and LAT2.  |  del Amo, EM., et al. 2008. Eur J Pharm Sci. 35: 161-74. PMID: 18656534
12. LAT2 regulates glutamine-dependent mTOR activation to promote glycolysis and chemoresistance in pancreatic cancer.  |  Feng, M., et al. 2018. J Exp Clin Cancer Res. 37: 274. PMID: 30419950