Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LAT CRISPR/Cas9 KO Plasmid (h): sc-401281
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LAT CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LAT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LAT Antibody (11B.12): sc-53550
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAT CRISPR/Cas9 KO Plasmid (h) | sc-401281 | 20 µg | $397.00 |
Overview
Linker for activation of T cells (LAT) is a transmembrane adaptor protein that becomes tyrosine-phosphorylated downstream of T cell receptor (TCR) engagement and acts as a scaffold to assemble multi-protein signaling complexes. LAT coordinates recruitment of GRB2, GADS, PLCγ1, and SOS to propagate signaling through calcium flux, Ras–MAPK/ERK, and NFAT/NF-κB pathways, shaping T cell activation, cytokine production, and immune synapse organization. In human cells, altered LAT-dependent signal integration is relevant to studies of immune dysregulation, including mechanisms that contribute to autoimmunity, immunodeficiency-like phenotypes, and aberrant lymphocyte activation programs. Because LAT sits at a critical node in proximal TCR signaling, it is widely used as a handle for dissecting pathway wiring, feedback control, and signal amplitude–duration encoding in adaptive immunity.
LAT CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LAT gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LAT together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LAT open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LAT protein expression.
This CRISPR knockout system enables efficient generation of LAT-deficient cell models for investigation of LAT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LAT exon(s) critical for LAT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LAT genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LAT CRISPR/Cas9 KO Plasmid (h) and LAT CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LAT locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LAT HDR Plasmid (h) and LAT HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LAT homology arms to support homology-directed repair at defined LAT target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.