LAT Antibody (F-7): sc-514182

LAT Antibody (F-7) is a mouse monoclonal IgG1 antibody that detects LAT in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAT (F-7) antibody recognizes the linker for activation of T cells, a crucial integral membrane protein that plays a significant role in T cell activation and immune response. LAT (F-7) monoclonal antibody targets a protein strategically located in the plasma membrane, where LAT serves as a critical signaling hub that facilitates the recruitment of various signaling molecules, including phospholipase C gamma 1, GRB2, and the p85 subunit of phosphoinositide 3-kinase. This localization is vital as LAT allows for rapid transmission of signals from the T cell receptor, leading to activation of downstream pathways that promote cell proliferation and differentiation. Furthermore, LAT undergoes palmitoylation, a post-translational modification essential for proper tyrosine phosphorylation and localization to lipid rafts, which are specialized microdomains in the membrane that enhance signaling efficiency. LAT′s role as a linker protein in T cell activation underscores its importance in orchestrating immune responses, making anti-LAT antibody (F-7) an invaluable tool for researchers studying T cell signaling and function.

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LAT Antibody (F-7) References:

1. Displacement of linker for activation of T cells from the plasma membrane due to redox balance alterations results in hyporesponsiveness of synovial fluid T lymphocytes in rheumatoid arthritis.  |  Gringhuis, SI., et al. 2000. J Immunol. 164: 2170-9. PMID: 10657671
2. Recruitment of SLP-76 to the membrane and glycolipid-enriched membrane microdomains replaces the requirement for linker for activation of T cells in T cell receptor signaling.  |  Boerth, NJ., et al. 2000. J Exp Med. 192: 1047-58. PMID: 11015445
3. LAT, the linker for activation of T cells: a bridge between T cell-specific and general signaling pathways.  |  Wange, RL. 2000. Sci STKE. 2000: re1. PMID: 11752630
4. Chromosomal localization and genomic organization of the human Linker for Activation of T cells (LAT) gene.  |  Windpassinger, C., et al. 2002. Cytogenet Genome Res. 97: 155-7. PMID: 12438705
5. Involvement of linker for activation of T cells in the costimulatory signaling pathways.  |  Tsuchida, M., et al. 2003. Transplant Proc. 35: 553-4. PMID: 12591528
6. Non-T cell activation linker promotes mast cell survival by dampening the recruitment of SHIP1 by linker for activation of T cells.  |  Roget, K., et al. 2008. J Immunol. 180: 3689-98. PMID: 18322174
7. Linker for activation of T cells contributes to airway inflammation in an asthmatic mouse model.  |  Guo, XJ., et al. 2010. Chin Med J (Engl). 123: 2676-81. PMID: 21034652
8. The linker for activation of T cells (LAT) signaling hub: from signaling complexes to microclusters.  |  Balagopalan, L., et al. 2015. J Biol Chem. 290: 26422-9. PMID: 26354432
9. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency.  |  Bacchelli, C., et al. 2017. J Allergy Clin Immunol. 139: 634-642.e5. PMID: 27522155
10. RNA-Sequencing Analysis and Expression of Lymphocyte-Specific Protein Tyrosine Kinase, Linker for Activation of T Cells, and 70-kDa T-Cell Receptor Zeta-Chain Associated Protein Kinase in Rat Liver Transplant Rejection.  |  Xuan, J., et al. 2023. Exp Clin Transplant. 21: 961-972. PMID: 38263783

The preferred LAT antibody is LAT Antibody (11B.12): sc-53550.