Laminin-R Antibody (G-7): sc-74531

Laminin-R Antibody (G-7) is a mouse monoclonal IgG1 antibody that detects Laminin-R in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Laminin-R, also known as colon carcinoma laminin-binding protein, plays a crucial role in cell adhesion and migration, which are essential processes in tissue development and repair, as well as in cancer metastasis. Laminin-R is characterized by its heterodimeric structure, which is similar to that of other extracellular matrix protein receptors, such as fibronectin and vitronectin. The incorporation of Laminin-R into lysosomal membranes facilitates the attachment of lysosomes to surfaces coated with laminin, highlighting Laminin-R′s importance in cellular interactions and signaling pathways. Notably, Laminin-R expression is significantly upregulated in aggressive carcinomas, particularly in colon cancer, where Laminin-R is found at nine-fold higher levels compared to adjacent normal colonic epithelium. This upregulation suggests that Laminin-R may serve as a potential biomarker for cancer progression and a target for therapeutic intervention. Additionally, the precursor of Laminin-R, identified as p40 ribosome-associated protein, is involved in the translational machinery, further underscoring the multifunctional nature of Laminin-R in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Laminin-R Antibody (G-7) References:

1. Inverse modulation of steady-state messenger RNA levels of two non-integrin laminin-binding proteins in human colon carcinoma.  |  Castronovo, V., et al. 1992. J Natl Cancer Inst. 84: 1161-9. PMID: 1386115
2. Anti-sense RNA of 32-kDa laminin-binding protein inhibits attachment and invasion of a human colon carcinoma cell line.  |  Mafune, K. and Ravikumar, TS. 1992. J Surg Res. 52: 340-6. PMID: 1534386
3. Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma.  |  Lee, EC., et al. 1991. Arch Surg. 126: 1498-502. PMID: 1842179
4. Identification of laminin binding proteins in cell membranes of a human colon adenocarcinoma cell line.  |  Stallmach, A., et al. 1990. Gut. 31: 70-6. PMID: 2138583
5. Expression of a Mr 32,000 laminin-binding protein messenger RNA in human colon carcinoma correlates with disease progression.  |  Mafune, K., et al. 1990. Cancer Res. 50: 3888-91. PMID: 2141294
6. Integrated analysis of gene expression and copy number variations in MET proto‑oncogene‑transformed human primary osteoblasts.  |  Jia, RJ., et al. 2018. Mol Med Rep. 17: 2543-2548. PMID: 29207108
7. Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering.  |  Mann, V., et al. 2019. Int J Mol Sci. 20: PMID: 30889841
8. Identification of key candidate genes involved in melanoma metastasis.  |  Chen, J., et al. 2019. Mol Med Rep. 20: 903-914. PMID: 31173190
9. Inhibition of macrophage migration inhibitory factor (MIF) as a therapeutic target in bleomycin-induced pulmonary fibrosis rats.  |  Luo, Y., et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321: L6-L16. PMID: 33881353
10. Onco-fetal/laminin-binding collagen from colon carcinoma: detection of new sequences.  |  Minafra, IP., et al. 1995. Biochem Biophys Res Commun. 207: 852-9. PMID: 7864881

The preferred Laminin-R antibody is Laminin-R Antibody (H-2): sc-74515.