Laminin-R Antibody (A-7): sc-376295

Laminin-R Antibody (A-7) is a mouse monoclonal IgG2b antibody that detects Laminin-R in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Laminin-R, also known as colon carcinoma laminin-binding protein, plays a crucial role in cell adhesion and migration, which are essential processes in tissue development and repair, as well as in cancer metastasis. Laminin-R is characterized by its heterodimeric structure, which is similar to that of other extracellular matrix protein receptors, such as fibronectin and vitronectin. The incorporation of Laminin-R into lysosomal membranes facilitates the attachment of lysosomes to surfaces coated with laminin, highlighting Laminin-R′s importance in cellular interactions and signaling pathways. Notably, Laminin-R expression is significantly upregulated in aggressive carcinomas, particularly in colon cancer, where Laminin-R is found at nine-fold higher levels compared to adjacent normal colonic epithelium. This upregulation suggests that Laminin-R may serve as a potential biomarker for cancer progression and a target for therapeutic intervention. Additionally, the precursor of Laminin-R, identified as p40 ribosome-associated protein, is involved in the translational machinery, further underscoring the multifunctional nature of Laminin-R in cellular processes.

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Laminin-R Antibody (A-7) References:

1. Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160.  |  Perrin, A., et al. 2017. Mol Ther Nucleic Acids. 6: 68-79. PMID: 28325301
2. Retinal Avascularity and Neovascularization Associated With LAMA1 (laminin1) Mutation in Poretti-Boltshauser Syndrome.  |  Marlow, E., et al. 2018. JAMA Ophthalmol. 136: 96-97. PMID: 29167897
3. Understanding What You Have Found: A Family With a Mutation in the LAMA1 Gene With Literature Review.  |  Elmas, M., et al. 2020. Clin Med Insights Case Rep. 13: 1179547620948666. PMID: 32884387
4. An Early Diagnostic Clue for COL18A1- and LAMA1-Associated Diseases: High Myopia With Alopecia Areata in the Cranial Midline.  |  Wang, P., et al. 2021. Front Cell Dev Biol. 9: 644947. PMID: 34249907
5. Identification of LAMA1 mutations ends diagnostic odyssey and has prognostic implications for patients with presumed Joubert syndrome.  |  Powell, L., et al. 2021. Brain Commun. 3: fcab163. PMID: 34423300
6. High myopia and vitreal veils in a patient with Poretti- Boltshauser syndrome due to a novel homozygous LAMA1 mutation.  |  Faizi, N., et al. 2022. Ophthalmic Genet. 43: 653-657. PMID: 35535551
7. Variability of retinopathy consequent upon novel mutations in LAMA1.  |  Schiff, ER., et al. 2022. Ophthalmic Genet. 43: 671-678. PMID: 35616092
8. Effects of experimental glaucoma in Lama1nmf223 mutant mice.  |  Madhoun, S., et al. 2023. Exp Eye Res. 226: 109341. PMID: 36476399
9. Association of LAMA1 Single-Nucleotide Polymorphisms with Risk of Esophageal Squamous Cell Carcinoma among the Eastern Chinese Population.  |  Zhang, S., et al. 2023. J Oncol. 2023: 6922909. PMID: 36824663
10. CRISPRa-induced upregulation of human LAMA1 compensates for LAMA2-deficiency in Merosin-deficient congenital muscular dystrophy.  |  Arockiaraj, AI., et al. 2023. bioRxiv.. PMID: 36945402

The preferred Laminin-R antibody is Laminin-R Antibody (H-2): sc-74515.