Laminin beta-3 Antibody (A-6) recommended for detection of Laminin β-3 of human origin by WB, IP, IF, IHC(P) and ELISA

Laminin β-3 Antibody (A-6): sc-133178

Laminin β-3 Antibody (A-6) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-Laminin β-3 Antibody (A-6) is a mouse monoclonal IgG1 (kappa light chain) Laminin β-3 antibody provided at 200 µg/ml
    • raised against amino acids 873-1172 mapping at the C-terminus of Laminin β-3 of human origin
    • Laminin beta-3 Antibody (A-6) is recommended for detection of Laminin β-3 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Laminin beta-3 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin β-3 (A-6): sc-133178.
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I am using Laminin β-3 (A-6): sc-133178 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from schwach Testsample erhalten, Probefärbungen durchgeführt, für unsere Zwecke nicht einsetzbar
Date published: 2017-08-28
Rated 5 out of 5 by from Clean bands with background Laminin β-3 antibody works very well for WB showing clear band with minimal background. This antibody has clean bands with background.
Date published: 2017-02-04
Rated 5 out of 5 by from Good western blot results We used this antibody for western blot and got a positive band for the correct molecular weight
Date published: 2017-01-17
Rated 5 out of 5 by from Positive result in WB in SCC Positive result in WB in SCC-4 whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Good signal for WB in human Laminin Good signal for WB in human Laminin -3 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-01-13
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