Laminin β-2 (m): 293T Lysate: sc-121282
- mouse Laminin β-2 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for Laminin β-2 antibodies
- Suitable control antibody: Laminin β-2 Antibody (C4): sc-59980 is recommended as control antibody for monitoring of Laminin β-2 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
QUICK LINKS
Laminin β-2 (m): 293T Lysate, derived from the HEK 293T cell line, is pivotal in researching the role of the laminin β-2 subunit in murine models, focusing on its involvement in cellular architecture and signaling pathways. The 293T cells, a variant of the original HEK 293 cells, have been modified to express the SV40 large T antigen, which enhances the replication and expression of recombinant proteins, making it suitable for the study of complex proteins like laminin β-2. This lysate facilitates the exploration of laminin β-2's integral role in the assembly and function of the basement membrane, particularly its interactions with other extracellular matrix components and receptors on the cell surface. Researchers utilize this lysate to dissect the mechanistic details of how laminin β-2 contributes to cellular adhesion, migration, and structural integrity, employing techniques such as immunocytochemistry, Western blotting, and integrin binding assays. This research provides crucial insights into the fundamental processes of cell-matrix interactions and the stabilization of cellular structures. By studying these mechanisms, scientists aim to explain the basic biological functions of laminin β-2, enhancing our understanding of tissue organization and cellular dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin β-2 (m): 293T Lysate | sc-121282 | 100 µg/200 µl | $236.00 |