Laminin alpha-4 Antibody (6C3) recommended for detection of Laminin α-4 of human origin by WB, IP and ELISA

Laminin α-4 Antibody (6C3): sc-130541

Laminin α-4 Antibody (6C3) is rated 3.0 out of 5 by 4.
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Datasheets

    • Anti-Laminin α-4 Antibody (6C3) is a mouse monoclonal IgG1 (kappa light chain) Laminin α-4 antibody provided at 100 µg/ml
    • raised against purified Laminin α4β1γ1 isolated from platelets of human origin
    • recommended for detection of Laminin α-4 of human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin α-4 (6C3): sc-130541.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Laminin α-4 (6C3): sc-130541 antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from Did not work for western blot Used a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No band observed.
Date published: 2017-11-30
Rated 4 out of 5 by from Staining of human RCC paraffin embedded tissue The antibody works well on human paraffin sections of renal cell carcinoma. Matching normal kidney tissue of the same RCC patient shows some Lama4 staining. Protocol After deparaffinization with xylene and ethanol block endogenous peroxidase with 0.3% hydrogen peroxidase/PBS 15 min at RT. Antigen retrieval with 10mM citrate buffer pH 6.0, block 5% BSA/PBS 1h at RT, anti-lama4 Ab dilution 1:30 in 0.5% BSA/PBS O.N. at 4C, 2nd Ab goat anti-mouse biotin (Dako, ref E0433) diution 1:500 in 0.5% BSA/PBS 45 min at RT. Strep-HRP (Dako, ref P0397) 1:200 in 0.5% BSA/PBS 30 min at RT. Develop with DAB, counterstain with Mayer's hematoxylin.
Date published: 2017-11-23
Rated 5 out of 5 by from Good results in IF of human kidney sections Staining with the antibody (1:100) resulted in good staining of murine kidney kryosections (PFA-fixed)
Date published: 2017-11-17
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