LAGE-1 Antibody (3H1): sc-517172
- LAGE-1 Antibody (3H1) is a mouse monoclonal IgG2b κ LAGE-1 antibody provided at 100 µg/ml
- raised against amino acids 111-210 representing partial length LAGE-1 of human origin
- recommended for detection of LAGE-1 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LAGE-1 Antibody (3H1). This work is in progress.
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LAGE-1 Antibody (3H1) is a mouse monoclonal IgG2b kappa light chain antibody that detects LAGE-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-LAGE-1 antibody (3H1) is available as the non-conjugated format. LAGE-1 plays a crucial role as a cancer-testis (CT) antigen, which is typically expressed in various malignancies while remaining absent in normal adult tissues, making LAGE-1 a promising target for immunotherapy. LAGE-1 (3H1) monoclonal antibody recognizes a protein known to activate type-1 melanoma-reactive CD4+ helper T cells, thereby facilitating robust and long-lasting antitumor immune responses. LAGE-1 gene encodes two distinct protein isoforms: a 180 amino acid variant featuring two glycine-rich regions and a hydrophobic stretch near the C-terminus, and a 210 amino acid variant that lacks this hydrophobic region. Both isoforms are predominantly expressed in melanoma cell lines, lung tumors, sarcomas, and other cancer types, while expression is restricted to the testis and placenta in normal tissues. This unique expression pattern underscores LAGE-1′s potential as a target for cancer immunotherapy, as LAGE-1 can elicit a specific immune response against tumor cells while sparing normal tissues.
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LAGE-1 Antibody (3H1) References:
- Seroreactivity against MAGE-A and LAGE-1 proteins in melanoma patients. | Usener, D., et al. 2003. Br J Dermatol. 149: 282-8. PMID: 12932233
- The alternative open reading frame of LAGE-1 gives rise to multiple promiscuous HLA-DR-restricted epitopes recognized by T-helper 1-type tumor-reactive CD4+ T cells. | Mandic, M., et al. 2003. Cancer Res. 63: 6506-15. PMID: 14559844
- Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number. | Sato, S., et al. 2005. Int J Oncol. 26: 57-63. PMID: 15586225
- Immunohistochemical and molecular analysis of human melanomas for expression of the human cancer-testis antigens NY-ESO-1 and LAGE-1. | Vaughan, HA., et al. 2004. Clin Cancer Res. 10: 8396-404. PMID: 15623618
- [Expression of NY-ESO-1/LAGE-1 genes in hepatocellular carcinoma and autologous humoral responses induced thereby]. | Xing, BC., et al. 2004. Zhonghua Yi Xue Za Zhi. 84: 1980-2. PMID: 15730810
- NY-ESO-1/LAGE-1 coexpression with MAGE-A cancer/testis antigens: a tissue microarray study. | Bolli, M., et al. 2005. Int J Cancer. 115: 960-6. PMID: 15751033
- [Expression of NY-ESO-1 and LAGE-1 cancer-testis antigens in hepatocellular carcinoma]. | Zhang, WM., et al. 2005. Zhonghua Bing Li Xue Za Zhi. 34: 202-5. PMID: 16091172
- A new LAGE-1 peptide recognized by cytolytic T lymphocytes on HLA-A68 tumors. | Sun, Z., et al. 2006. Cancer Immunol Immunother. 55: 644-52. PMID: 16187088
- LAGE-1, a new gene with tumor specificity. | Lethé, B., et al. 1998. Int J Cancer. 76: 903-8. PMID: 9626360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAGE-1 Antibody (3H1) | sc-517172 | 100 µg/ml | $322.00 |