L-Myc CRISPR/Cas9 KO Plasmid (h): sc-401836
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-Myc CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-Myc genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-Myc CRISPR/Cas9 KO Plasmid (h) | sc-401836 | 20 µg | $397.00 |
Overview
MYCL encodes L-Myc, a basic helix–loop–helix leucine zipper transcription factor in the MYC family that heterodimerizes with MAX to regulate RNA polymerase II–driven gene expression. L-Myc influences cell-cycle progression, ribosome biogenesis, metabolic reprogramming, and chromatin-associated transcriptional control, integrating signals across proliferation and differentiation programs. MYCL activity intersects with MYC/MAX network dynamics and can modulate E2F-dependent transcription and apoptotic stress responses depending on cellular context. Dysregulation or amplification of MYCL is linked to oncogenic transcriptional programs in select tumor types, making it a useful node for studying lineage-associated growth control and transcription factor circuitry.
L-Myc CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYCL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYCL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYCL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-Myc protein expression.
This CRISPR knockout system enables efficient generation of MYCL-deficient cell models for investigation of L-Myc signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYCL exon(s) critical for L-Myc function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYCL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-Myc CRISPR/Cas9 KO Plasmid (h) and L-Myc CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYCL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-Myc HDR Plasmid (h) and L-Myc HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYCL homology arms to support homology-directed repair at defined MYCL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.