L-ficolin Antibody (FCN219): sc-130297
- L-ficolin Antibody (FCN219) is a mouse monoclonal IgG2a κ L-ficolin antibody, cited in 2 publications, provided at 200 µg/ml
- raised against recombinant L-ficolin of human origin
- L-ficolin Antibody (FCN219) is recommended for detection of L-ficolin of human origin and ficolin B of mouse and rat origin by WB, IP and ELISA; non cross-reactive with M-ficolin or H-ficolin
- Anti-L-ficolin Antibody (FCN219) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for L-ficolin Antibody (FCN219). This work is in progress.
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L-ficolin Antibody (FCN219) is a mouse monoclonal IgG2a kappa light chain antibody that detects L-ficolin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-L-ficolin antibody (FCN219) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. L-ficolin, also known as ficolin-2 or ficolin-B in mouse and rat, is a 313 amino acid protein that plays a crucial role in innate immune response as a pattern recognition molecule. L-ficolin is primarily secreted by the liver and found in plasma, where L-ficolin forms a disulfide-linked homopolymer, which can lead to the formation of a functional dodecamer polypeptide. This unique structure is essential for L-ficolin′s ability to bind to DNA ligands expressed by late apoptotic and necrotic cells, enhancing their attachment and subsequent engulfment by immune cells. L-ficolin concentration can vary significantly among individuals due to polymorphisms in the promoter and structural regions of the FCN2 gene, which has been linked to various health conditions, including Behçet′s disease. In patients with Behçet′s disease, notable differences in allele frequency for single nucleotide polymorphisms within the FCN2 gene have been observed, particularly at the -557 and -64 promoter sites, highlighting L-ficolin′s importance in immune regulation and disease susceptibility.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
L-ficolin Antibody (FCN219) References:
- L-ficolin specifically binds to lipoteichoic acid, a cell wall constituent of Gram-positive bacteria, and activates the lectin pathway of complement. | Lynch, NJ., et al. 2004. J Immunol. 172: 1198-202. PMID: 14707097
- Human mannose-binding lectin and L-ficolin function as specific pattern recognition proteins in the lectin activation pathway of complement. | Ma, YG., et al. 2004. J Biol Chem. 279: 25307-12. PMID: 15078867
- L-ficolin is a pattern recognition molecule specific for acetyl groups. | Krarup, A., et al. 2004. J Biol Chem. 279: 47513-9. PMID: 15331601
- Role of L-ficolin/mannose-binding lectin-associated serine protease complexes in the opsonophagocytosis of type III group B streptococci. | Aoyagi, Y., et al. 2005. J Immunol. 174: 418-25. PMID: 15611266
- Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-ficolin, and H-ficolin. | Krarup, A., et al. 2005. Infect Immun. 73: 1052-60. PMID: 15664949
- Specific binding of L-ficolin and H-ficolin to apoptotic cells leads to complement activation. | Kuraya, M., et al. 2005. Immunobiology. 209: 689-97. PMID: 15804047
- Coding and non-coding polymorphisms in the lectin pathway activator L-ficolin gene in 188 Dutch blood bank donors. | Herpers, BL., et al. 2006. Mol Immunol. 43: 851-5. PMID: 16076493
- Development of a fluorescence assay for the detection of L-ficolin-MASP in serum or purified samples. | Gulla, KC., et al. 2006. J Biochem Biophys Methods. 66: 59-71. PMID: 16442165
- Localization of the mouse defense lectin ficolin B in lysosomes of activated macrophages. | Runza, VL., et al. 2006. J Endotoxin Res. 12: 120-6. PMID: 16690015
- Structural insights into the innate immune recognition specificities of L- and H-ficolins. | Garlatti, V., et al. 2007. EMBO J. 26: 623-33. PMID: 17215869
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-ficolin Antibody (FCN219) | sc-130297 | 200 µg/ml | $322.00 | |||
L-ficolin Antibody (FCN219) AC | sc-130297 AC | 500 µg/ml, 25% agarose | $424.00 | |||
L-ficolin Antibody (FCN219) HRP | sc-130297 HRP | 200 µg/ml | $322.00 | |||
L-ficolin Antibody (FCN219) FITC | sc-130297 FITC | 200 µg/ml | $336.00 | |||
L-ficolin Antibody (FCN219) PE | sc-130297 PE | 200 µg/ml | $349.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 488 | sc-130297 AF488 | 200 µg/ml | $364.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 546 | sc-130297 AF546 | 200 µg/ml | $364.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 594 | sc-130297 AF594 | 200 µg/ml | $364.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 647 | sc-130297 AF647 | 200 µg/ml | $364.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 680 | sc-130297 AF680 | 200 µg/ml | $364.00 | |||
L-ficolin Antibody (FCN219) Alexa Fluor® 790 | sc-130297 AF790 | 200 µg/ml | $364.00 |