KV6.3 CRISPR/Cas9 KO Plasmid (h): sc-413962
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KV6.3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KV6.3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KV6.3 CRISPR/Cas9 KO Plasmid (h) | sc-413962 | 20 µg | $397.00 |
Overview
KCNG4 encodes the Kv6.3 subunit, an electrically silent member of the voltage-gated potassium channel family that modulates membrane excitability by forming heterotetramers with Kv2 channel α-subunits. Through this modulatory role, Kv6.3 influences action potential waveform, firing thresholds, and repolarization kinetics, integrating into ion homeostasis and excitability-associated signaling processes. KCNG4 expression has been reported in excitable tissues and is relevant to pathways governing neuronal and sensory signaling as well as smooth muscle electrical behavior. Dysregulation of potassium channel modulators is broadly implicated in disorders of excitability, supporting KCNG4 as a target for mechanistic studies of ion channel composition and function in disease-relevant cellular models.
KV6.3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNG4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNG4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNG4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KV6.3 protein expression.
This CRISPR knockout system enables efficient generation of KCNG4-deficient cell models for investigation of KV6.3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNG4 exon(s) critical for KV6.3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNG4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KV6.3 CRISPR/Cas9 KO Plasmid (h) and KV6.3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNG4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KV6.3 HDR Plasmid (h) and KV6.3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNG4 homology arms to support homology-directed repair at defined KCNG4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.