KV1.3 CRISPR/Cas9 KO Plasmid (m): sc-421214
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KV1.3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KV1.3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KV1.3 Antibody (G-9): sc-398855
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KV1.3 CRISPR/Cas9 KO Plasmid (m) | sc-421214 | 20 µg | $397.00 |
Overview
Kcna3 encodes the voltage-gated potassium channel KV1.3, a Shaker-family channel that regulates membrane potential and potassium efflux to shape calcium signaling, activation thresholds, and electrical excitability. In immune and glial cells, KV1.3 activity influences signaling cascades linked to proliferation, cytokine production, and metabolic reprogramming by controlling Ca2+-dependent transcriptional programs. Channel function intersects with pathways governing cell-cycle progression and stress responses, and altered KV1.3 activity has been associated with immune dysregulation and inflammatory mechanisms. In the nervous system, KV1.3 contributes to excitability and signaling dynamics relevant to neuroinflammation and neuronal-glial communication.
KV1.3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Kcna3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Kcna3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Kcna3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KV1.3 protein expression.
This CRISPR knockout system enables efficient generation of Kcna3-deficient cell models for investigation of KV1.3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Kcna3 exon(s) critical for KV1.3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Kcna3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KV1.3 CRISPR/Cas9 KO Plasmid (m) and KV1.3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Kcna3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KV1.3 HDR Plasmid (m) and KV1.3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Kcna3 homology arms to support homology-directed repair at defined Kcna3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.