KPI-2 Antibody (D-11): sc-398396
- KPI-2 Antibody (D-11) is a mouse monoclonal IgG1 κ KPI-2 antibody provided at 200 µg/ml
- raised against amino acids 15-148 mapping near the N-terminus of KPI-2 of human origin
- KPI-2 Antibody (D-11) is recommended for detection of KPI-2 of human origin by WB, IP, IF and ELISA
- Anti-KPI-2 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for KPI-2 Antibody (D-11) for WB applications. These reagents are now offered in bundles with KPI-2 Antibody (D-11) (see ordering information below).
QUICK LINKS
KPI-2 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects KPI-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KPI-2 (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KPI-2, also known as kinase/phosphatase/inhibitor 2, BREK, LMR2, cprk, AATYK2, or lemur tyrosine kinase 2 (LMTK2), is a 1,503 amino acid single-pass membrane protein that belongs to the protein kinase superfamily and protein tyrosine kinase family. KPI-2 is primarily expressed in skeletal muscle, with weaker expression in brain and pancreas. KPI-2 features N-terminal transmembrane helices and a long C-terminal cytoplasmic tail that exhibits serine/threonine/tyrosine kinase activity. KPI-2 plays a crucial role in nerve growth factor (NGF)-TrkA signaling, endosomal membrane trafficking, and spermatogenesis, and is essential for transitioning endocytosed membrane vesicles from early endosomes to recycling endosomes. KPI-2 localizes to cytoplasmic membrane vesicles and perinuclear recycling endosomes, underscoring KPI-2′s importance in cellular transport processes. Additionally, KPI-2 has emerged as a potential therapeutic target for prostate cancer, highlighting KPI-2′s significance in cancer research and treatment strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KPI-2 Antibody (D-11) References:
- A novel transmembrane Ser/Thr kinase complexes with protein phosphatase-1 and inhibitor-2. | Wang, H. and Brautigan, DL. 2002. J Biol Chem. 277: 49605-12. PMID: 12393858
- Involvement of BREK, a serine/threonine kinase enriched in brain, in NGF signalling. | Kawa, S., et al. 2004. Genes Cells. 9: 219-32. PMID: 15005709
- Peptide microarray analysis of substrate specificity of the transmembrane Ser/Thr kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase. | Wang, H. and Brautigan, DL. 2006. Mol Cell Proteomics. 5: 2124-30. PMID: 16887929
- Azoospermia in mice with targeted disruption of the Brek/Lmtk2 (brain-enriched kinase/lemur tyrosine kinase 2) gene. | Kawa, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 19344-9. PMID: 17158803
- Myosin VI and its interacting protein LMTK2 regulate tubule formation and transport to the endocytic recycling compartment. | Chibalina, MV., et al. 2007. J Cell Sci. 120: 4278-88. PMID: 18029400
- Multiple newly identified loci associated with prostate cancer susceptibility. | Eeles, RA., et al. 2008. Nat Genet. 40: 316-21. PMID: 18264097
- BREK/LMTK2 is a myosin VI-binding protein involved in endosomal membrane trafficking. | Inoue, T., et al. 2008. Genes Cells. 13: 483-95. PMID: 18429820
- Identification of new genetic risk factors for prostate cancer. | Guy, M., et al. 2009. Asian J Androl. 11: 49-55. PMID: 19050691
- LMTK2 and PARP-2 gene polymorphism and azoospermia secondary to meiotic arrest. | Sakugawa, N., et al. 2009. J Assist Reprod Genet. 26: 545-52. PMID: 19806447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KPI-2 Antibody (D-11) | sc-398396 | 200 µg/ml | $322.00 | |||
KPI-2 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-527771 | 200 µg Ab; 10 µg BP | $361.00 | |||
KPI-2 Antibody (D-11): m-IgG1 BP-HRP Bundle | sc-533144 | 200 µg Ab; 20 µg BP | $361.00 | |||
KPI-2 Antibody (D-11) AC | sc-398396 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KPI-2 Antibody (D-11) HRP | sc-398396 HRP | 200 µg/ml | $322.00 | |||
KPI-2 Antibody (D-11) FITC | sc-398396 FITC | 200 µg/ml | $336.00 | |||
KPI-2 Antibody (D-11) PE | sc-398396 PE | 200 µg/ml | $349.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 488 | sc-398396 AF488 | 200 µg/ml | $364.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 546 | sc-398396 AF546 | 200 µg/ml | $364.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 594 | sc-398396 AF594 | 200 µg/ml | $364.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 647 | sc-398396 AF647 | 200 µg/ml | $364.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 680 | sc-398396 AF680 | 200 µg/ml | $364.00 | |||
KPI-2 Antibody (D-11) Alexa Fluor® 790 | sc-398396 AF790 | 200 µg/ml | $364.00 |