KLRG1 CRISPR/Cas9 KO Plasmid (h): sc-409220
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KLRG1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KLRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLRG1 CRISPR/Cas9 KO Plasmid (h) | sc-409220 | 20 µg | $397.00 |
Overview
KLRG1 (killer cell lectin like receptor G1) is an inhibitory receptor expressed on subsets of human NK cells and effector/memory T cells, where it helps calibrate cytotoxicity and cytokine production during immune surveillance. Through binding to cadherins such as E-cadherin, KLRG1 transduces inhibitory signaling that influences activation thresholds, cellular differentiation, and features of immune exhaustion and senescence. KLRG1 is widely used as a marker to phenotype terminally differentiated lymphocyte populations and to track antigen-driven responses in chronic infection, inflammation, and tumor microenvironments. Dysregulated KLRG1-associated inhibitory tone is therefore relevant to studies of immune suppression, tissue residency, and checkpoint-like pathways shaping anti-viral and anti-tumor immunity.
KLRG1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KLRG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KLRG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KLRG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KLRG1 protein expression.
This CRISPR knockout system enables efficient generation of KLRG1-deficient cell models for investigation of KLRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KLRG1 exon(s) critical for KLRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KLRG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KLRG1 CRISPR/Cas9 KO Plasmid (h) and KLRG1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KLRG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KLRG1 HDR Plasmid (h) and KLRG1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KLRG1 homology arms to support homology-directed repair at defined KLRG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.