KLHL9/13 Antibody (D-4): sc-166486

KLHL9/13 Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects KLHL9/13 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KLHL9/13 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KLHL9, consisting of 617 amino acids, features a BACK (BTB/kelch associated) domain, six kelch repeats, and a BTB/POZ domain, while KLHL13, also known as BKLHD2, is a 604 amino acid protein with similar structural characteristics. These proteins serve as substrate-specific adapters for an E3 ubiquitin-protein ligase complex with CUL-3 in the ubiquitin-proteasome system. Such interaction enables E3 ligases to transfer ubiquitin from E2 ubiquitin-conjugating enzymes to target proteins, marking them for degradation. KLHL9 and KLHL13′s ability to modulate protein ubiquitination maintains cellular homeostasis and regulates various biological processes, including cell cycle progression and stress response.

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KLHL9/13 Antibody (D-4) References:

1. One ring to rule a superfamily of E3 ubiquitin ligases.  |  Tyers, M. and Willems, AR. 1999. Science. 284: 601, 603-4. PMID: 10328744
2. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.  |  Singer, JD., et al. 1999. Genes Dev. 13: 2375-87. PMID: 10500095
3. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 2000. DNA Res. 7: 65-73. PMID: 10718198
4. Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20.  |  Morrow, CJ., et al. 2005. J Cell Sci. 118: 3639-52. PMID: 16046481
5. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells.  |  Sumara, I., et al. 2007. Dev Cell. 12: 887-900. PMID: 17543862
6. A Cul3-based E3 ligase regulates mitosis and is required to maintain the spindle assembly checkpoint in human cells.  |  Sumara, I. and Peter, M. 2007. Cell Cycle. 6: 3004-10. PMID: 18075312
7. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis.  |  Maerki, S., et al. 2009. J Cell Biol. 187: 791-800. PMID: 19995937
8. Deficiency of the autophagy gene ATG16L1 induces insulin resistance through KLHL9/KLHL13/CUL3-mediated IRS1 degradation.  |  Frendo-Cumbo, S., et al. 2019. J Biol Chem. 294: 16172-16185. PMID: 31515271
9. Significance of Chr9p22.1-p21.3 Deletion in Cancer Development: A Pan-cancer In Silico Analysis.  |  Gonçalves, PG., et al. 2022. Anticancer Res. 42: 5291-5304. PMID: 36288884
10. Defining E3 ligase-substrate relationships through multiplex CRISPR screening.  |  Timms, RT., et al. 2023. Nat Cell Biol. 25: 1535-1545. PMID: 37735597
11. Autophagy gene-dependent intracellular immunity triggered by interferon-γ.  |  McAllaster, MR., et al. 2023. mBio. 14: e0233223. PMID: 37905813
12. The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation.  |  Yao, Y., et al. 2025. Cell Rep. 44: 115101. PMID: 39708321

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KLHL9/13 Antibody (D-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-KLHL9/13 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KLHL9/13 Antibody (D-4) Specifications

Alternate Product Name: KLHL9/13

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 329-614

Epitope Target: KLHL9

Epitope Species: human

Product Reactivity/Detection: KLHL9 and KLHL13

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for KLHL9/13 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin