KLHL5 Antibody (A-2): sc-515745

KLHL5 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects KLHL5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KLHL5 (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KLHL5, also known as kelch-like protein 5, is a 755 amino acid protein that shares structural similarities with the Drosophila kelch protein and is primarily localized to the cytoskeleton. This localization plays a significant role in maintaining the integrity and organization of the cytoskeletal framework, which is essential for various cellular processes, including cell shape, motility, and division. KLHL5 is expressed at higher levels in the thyroid gland, adrenal gland, and ovary, while showing lower expression in the spinal cord, prostate, lymph node, trachea, and testis. KLHL5 features six kelch repeats and one BTB (broad complex, tramtrack, and bric-a-brac) domain, which facilitates homodimerization and is implicated in transcriptional regulation through chromatin structure and function modulation. KLHL5 is produced in three isoforms due to alternative splicing, further diversifying functional roles in cellular processes.

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KLHL5 Antibody (A-2) References:

1. The kelch repeat superfamily of proteins: propellers of cell function.  |  Adams, J., et al. 2000. Trends Cell Biol. 10: 17-24. PMID: 10603472
2. In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions.  |  Melnick, A., et al. 2000. Mol Cell Biol. 20: 6550-67. PMID: 10938130
3. Cloning and characterization of KLHL5, a novel human gene encoding a kelch-related protein with a BTB domain.  |  Wang, S., et al. 2001. Biochem Genet. 39: 227-38. PMID: 11590829
4. Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals.  |  Prag, S. and Adams, JC. 2003. BMC Bioinformatics. 4: 42. PMID: 13678422
5. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.  |  Geyer, R., et al. 2003. Mol Cell. 12: 783-90. PMID: 14527422
6. Characterization of a novel splicing variant of KLHL5, a member of the kelch protein family.  |  Xu, J., et al. 2003. Mol Biol Rep. 30: 239-42. PMID: 14672410
7. KLHL5 knockdown increases cellular sensitivity to anticancer drugs.  |  Schleifer, RJ., et al. 2018. Oncotarget. 9: 37429-37438. PMID: 30647843
8. Kelch-like protein 5-mediated ubiquitination of lysine 183 promotes proteasomal degradation of sphingosine kinase 1.  |  Powell, JA., et al. 2019. Biochem J. 476: 3211-3226. PMID: 31652307
9. KLHL5 Is a Prognostic-Related Biomarker and Correlated With Immune Infiltrates in Gastric Cancer.  |  Wu, Q., et al. 2020. Front Mol Biosci. 7: 599110. PMID: 33363208
10. miR-181-5p/KLHL5 Promoted Proliferation and Migration of Gastric Cancer Through Activating METTL3-Mediated m6A Process.  |  Li, R., et al. 2024. Mol Biotechnol. 66: 2415-2425. PMID: 37733183
11. The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins.  |  Albagli, O., et al. 1995. Cell Growth Differ. 6: 1193-8. PMID: 8519696
12. Drosophila kelch is an oligomeric ring canal actin organizer.  |  Robinson, DN. and Cooley, L. 1997. J Cell Biol. 138: 799-810. PMID: 9265647

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KLHL5 Antibody (A-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-KLHL5 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KLHL5 Antibody (A-2) Specifications

Alternate Product Name: KLHL5

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 58-144

Epitope Target: KLHL5

Epitope Species: human

Product Reactivity/Detection: KLHL5 isoforms 1-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for KLHL5 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin