KLF8 Antibody (12D2): sc-134375

KLF8 Antibody (12D2) is a mouse monoclonal IgG2a kappa light chain antibody that detects KLF8 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-KLF8 antibody (12D2) is available as a non-conjugated monoclonal isotype antibody. Krüppel-like factor 8 (KLF8), also known as basic Krüppel-like factor 3 or zinc finger protein 741, is a crucial transcriptional repressor composed of 359 amino acids that plays a significant role in regulating gene expression by binding to CACCC elements in DNA. KLF8 is predominantly located in the nucleus of various cell types, where KLF8 is involved in developmental processes and is notably upregulated in several human cancers, highlighting KLF8′s importance in tumorigenesis. KLF8′s ability to modulate gene expression is further influenced by post-translational modifications, particularly sumoylation, which serves as a regulatory mechanism. The modification of KLF8 at the sumoylation site, specifically the mutation of Lysine 67 to Arginine 67, has been shown to enhance transcriptional activity, thereby affecting KLF8′s role in cancer progression and cellular differentiation. KLF8′s versatility in gene regulation underscores potential as a therapeutic target and biomarker in cancer research.

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KLF8 Antibody (12D2) References:

1. Human Krüppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.  |  van Vliet, J., et al. 2000. Nucleic Acids Res. 28: 1955-62. PMID: 10756197
2. Abnormal expression of the KLF8 (ZNF741) gene in a female patient with an X;autosome translocation t(X;21)(p11.2;q22.3) and non-syndromic mental retardation.  |  Lossi, AM., et al. 2002. J Med Genet. 39: 113-7. PMID: 11836360
3. Identification of transcription factor KLF8 as a downstream target of focal adhesion kinase in its regulation of cyclin D1 and cell cycle progression.  |  Zhao, J., et al. 2003. Mol Cell. 11: 1503-15. PMID: 12820964
4. Cell and tissue specific expression of human Krüppel-like transcription factors in human ocular surface.  |  Chiambaretta, F., et al. 2004. Mol Vis. 10: 901-9. PMID: 15570219
5. Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation.  |  Wei, H., et al. 2006. J Biol Chem. 281: 16664-71. PMID: 16617055
6. New concepts regarding focal adhesion kinase promotion of cell migration and proliferation.  |  Cox, BD., et al. 2006. J Cell Biochem. 99: 35-52. PMID: 16823799
7. KLF8 transcription factor participates in oncogenic transformation.  |  Wang, X. and Zhao, J. 2007. Oncogene. 26: 456-61. PMID: 16832343
8. Systematic RNAi studies on the role of Sp/KLF factors in globin gene expression and erythroid differentiation.  |  Hu, JH., et al. 2007. J Mol Biol. 366: 1064-73. PMID: 17224162