KLF6 Double Nickase Plasmid (h): sc-400888-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KLF6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KLF6 Double Nickase Plasmid (h) and KLF6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLF6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KLF6 Antibody (E-10): sc-365633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLF6 Double Nickase Plasmid (h) | sc-400888-NIC | 20 µg | $410.00 | |||
KLF6 Double Nickase Plasmid (h2) | sc-400888-NIC-2 | 20 µg | $410.00 |
KLF6 (Krüppel-like factor 6) encodes a zinc-finger transcription factor that binds GC-rich promoter elements to regulate genes controlling cell-cycle progression, differentiation, and responses to cellular stress. It participates in transcriptional programs intersecting with p53-dependent checkpoints and TGF-β–associated signaling, shaping context-dependent control of proliferation and apoptosis. Altered KLF6 activity or expression has been linked to disrupted growth regulation and inflammation-associated phenotypes, making it a relevant node for studying transcriptional dysregulation in human disease biology. In biomedical research, KLF6 is commonly investigated for its roles in tumor suppressor–like functions, tissue remodeling, and gene regulatory network stability.
KLF6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLF6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLF6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLF6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLF6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.