kleisin β Antibody (B-1): sc-393333
- kleisin β Antibody (B-1) is a mouse monoclonal IgG1 κ kleisin β antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 437-606 mapping at the C-terminus of kleisin β of human origin
- kleisin beta Antibody (B-1) is recommended for detection of kleisin β of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-kleisin beta Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for kleisin β Antibody (B-1) for WB applications. This reagent is now offered in a bundle with kleisin β Antibody (B-1) (see ordering information below).
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kleisin β Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects kleisin beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). kleisin β Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. kleisin beta (NCAPH2) is a critical component of the condensin II complex, which plays a vital role in the organization and segregation of chromosomes during cell division. NCAPH2 localizes to the nucleus and is distributed along the arms of assembled chromosomes, where NCAPH2 contributes to the structural integrity and rigidity necessary for proper mitotic chromosome architecture. This function is essential for ensuring accurate chromosome segregation, which is crucial for maintaining genomic stability. NCAPH2 undergoes post-translational modifications, such as phosphorylation by ATM or ATR in response to DNA damage, highlighting NCAPH2′s role in the cellular response to genomic stress. Multiple isoforms of NCAPH2 due to alternative splicing further underscores the complexity and regulatory potential of this protein in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
kleisin β Antibody (B-1) References:
- Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners. | Schleiffer, A., et al. 2003. Mol Cell. 11: 571-5. PMID: 12667442
- Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells. | Ono, T., et al. 2003. Cell. 115: 109-21. PMID: 14532007
- Reconstitution and subunit geometry of human condensin complexes. | Onn, I., et al. 2007. EMBO J. 26: 1024-34. PMID: 17268547
- A mutation in a chromosome condensin II subunit, kleisin beta, specifically disrupts T cell development. | Gosling, KM., et al. 2007. Proc Natl Acad Sci U S A. 104: 12445-50. PMID: 17640884
- Defective T-cell function leading to reduced antibody production in a kleisin-beta mutant mouse. | Gosling, KM., et al. 2008. Immunology. 125: 208-17. PMID: 18397266
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
kleisin β Antibody (B-1) | sc-393333 | 200 µg/ml | $322.00 | |||
kleisin β Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-531758 | 200 µg Ab; 20 µg BP | $361.00 | |||
kleisin β Antibody (B-1) AC | sc-393333 AC | 500 µg/ml, 25% agarose | $424.00 | |||
kleisin β Antibody (B-1) HRP | sc-393333 HRP | 200 µg/ml | $322.00 | |||
kleisin β Antibody (B-1) FITC | sc-393333 FITC | 200 µg/ml | $336.00 | |||
kleisin β Antibody (B-1) PE | sc-393333 PE | 200 µg/ml | $349.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 488 | sc-393333 AF488 | 200 µg/ml | $364.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 546 | sc-393333 AF546 | 200 µg/ml | $364.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 594 | sc-393333 AF594 | 200 µg/ml | $364.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 647 | sc-393333 AF647 | 200 µg/ml | $364.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 680 | sc-393333 AF680 | 200 µg/ml | $364.00 | |||
kleisin β Antibody (B-1) Alexa Fluor® 790 | sc-393333 AF790 | 200 µg/ml | $364.00 |