KIR2.3 CRISPR/Cas9 KO Plasmid (h): sc-406604
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIR2.3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIR2.3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIR2.3 CRISPR/Cas9 KO Plasmid (h) | sc-406604 | 20 µg | $397.00 |
Overview
KCNJ4 encodes the inwardly rectifying potassium channel KIR2.3, a tetrameric membrane protein that preferentially conducts K+ at hyperpolarized potentials to stabilize resting membrane potential and shape cellular excitability. KIR2.3 activity contributes to potassium homeostasis and electrical signaling, influencing processes such as action potential repolarization and excitability coupling in excitable tissues. As part of the Kir2.x family, it interfaces functionally with pathways that depend on membrane polarization, including Ca2+-dependent signaling and stimulus-secretion coupling. Altered inward rectifier channel function is relevant to studies of electrophysiology, arrhythmogenic mechanisms, and excitable-cell dysfunction, making KCNJ4 a useful target for dissecting ion-channel contributions to cellular phenotypes.
KIR2.3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNJ4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNJ4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNJ4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIR2.3 protein expression.
This CRISPR knockout system enables efficient generation of KCNJ4-deficient cell models for investigation of KIR2.3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNJ4 exon(s) critical for KIR2.3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNJ4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIR2.3 CRISPR/Cas9 KO Plasmid (h) and KIR2.3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNJ4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIR2.3 HDR Plasmid (h) and KIR2.3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNJ4 homology arms to support homology-directed repair at defined KCNJ4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.