KIF2A Double Nickase Plasmid (h): sc-405146-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIF2A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KIF2A Double Nickase Plasmid (h) and KIF2A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KIF2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KIF2A Antibody (D-7): sc-271471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIF2A Double Nickase Plasmid (h) | sc-405146-NIC | 20 µg | $410.00 | |||
KIF2A Double Nickase Plasmid (h2) | sc-405146-NIC-2 | 20 µg | $410.00 |
KIF2A encodes a kinesin-13 family microtubule depolymerase that regulates microtubule plus-end dynamics, spindle assembly, and chromosome segregation during mitosis, supporting accurate cell cycle progression. In post-mitotic contexts, KIF2A contributes to axon guidance, neuronal migration, and synaptic development by remodeling microtubule arrays and coordinating cytoskeletal organization. Functional links place KIF2A within microtubule-based transport and mitotic checkpoint-associated processes that shape genome stability and cellular polarity. Dysregulated KIF2A activity has been associated with neurodevelopmental phenotypes and is frequently studied in cancer-relevant settings where altered microtubule dynamics influence proliferation and aneuploidy.
KIF2A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KIF2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KIF2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KIF2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KIF2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.