Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KIF14 CRISPR/Cas9 KO Plasmid (h): sc-408805
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIF14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIF14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KIF14 Antibody (E-3): sc-365553
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIF14 CRISPR/Cas9 KO Plasmid (h) | sc-408805 | 20 µg | $397.00 |
Overview
KIF14 encodes a kinesin-3 family microtubule motor that localizes to the mitotic spindle and midbody to coordinate chromosome segregation and cytokinesis. It supports cell-cycle progression by regulating spindle dynamics, central spindle organization, and completion of abscission, linking microtubule-based transport to late mitotic events. Aberrant KIF14 expression or activity is frequently associated with altered proliferative capacity, aneuploidy, and genomic instability in cancer biology. As a result, KIF14 is commonly studied in pathways governing mitotic fidelity, cytoskeletal remodeling, and proliferation-associated transcriptional programs.
KIF14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIF14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIF14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIF14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIF14 protein expression.
This CRISPR knockout system enables efficient generation of KIF14-deficient cell models for investigation of KIF14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIF14 exon(s) critical for KIF14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIF14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIF14 CRISPR/Cas9 KO Plasmid (h) and KIF14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIF14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIF14 HDR Plasmid (h) and KIF14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIF14 homology arms to support homology-directed repair at defined KIF14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.