KIAA1731 CRISPR/Cas9 KO Plasmid (h): sc-406955
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA1731 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIAA1731 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA1731 CRISPR/Cas9 KO Plasmid (h) | sc-406955 | 20 µg | $397.00 |
Overview
CEP295 (KIAA1731) encodes a centrosomal protein essential for centriole assembly and centrosome maturation, supporting the formation of the pericentriolar material and proper microtubule-organizing center function. It participates in processes that coordinate centriole-to-centrosome conversion, centrosome duplication, and faithful spindle formation during mitosis, thereby contributing to genome stability. Disruption of CEP295-dependent centrosome integrity can lead to aberrant mitotic progression and chromosomal instability, cellular states frequently studied in cancer biology. CEP295 is also relevant to investigations of ciliogenesis and cell-cycle control, where centrosome defects can alter signaling dynamics and differentiation programs.
KIAA1731 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEP295 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEP295 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEP295 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIAA1731 protein expression.
This CRISPR knockout system enables efficient generation of CEP295-deficient cell models for investigation of KIAA1731 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CEP295 exon(s) critical for KIAA1731 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CEP295 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIAA1731 CRISPR/Cas9 KO Plasmid (h) and KIAA1731 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEP295 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIAA1731 HDR Plasmid (h) and KIAA1731 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEP295 homology arms to support homology-directed repair at defined CEP295 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.